EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:10006

Smad2 MAD homolog 2 (Drosophila) ( MGI:108051)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_000185. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_000185_01	euxassay_000185_02	euxassay_000185_03	euxassay_000185_04

euxassay_000185_05	euxassay_000185_06	euxassay_000185_07	euxassay_000185_08	euxassay_000185_09

euxassay_000185_10	euxassay_000185_11	euxassay_000185_12	euxassay_000185_13	euxassay_000185_14

euxassay_000185_15	euxassay_000185_16	euxassay_000185_17	euxassay_000185_18	euxassay_000185_19

euxassay_000185_20	euxassay_000185_21	euxassay_000185_22	euxassay_000185_23	euxassay_000185_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10006_wholemount_strong.wlz

10006_wholemount_moderate.wlz

10006_wholemount_weak.wlz

10006_wholemount_possible.wlz

10006_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10006_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

medulla oblongata basal plate mantle layer

strong

regional

strong expression: see section 08 09 13 17 moderate expression: see section 16

midbrain roof plate

strong

single cell

strong expression: see section 13 14

tegmentum

strong

regional

strong expression: see section 10 11 13 14

facial vii ganglion

strong

homogeneous

strong expression: see section 06 19 20 21 moderate expression: see section 05 07

glossopharyngeal ix ganglion

strong

homogeneous

strong expression: see section 06 18 19 20 moderate expression: see section 07

trigeminal v ganglion

strong

homogeneous

strong expression: see section 03 04 05 06 07 08 09 17 18 19 20 21 22

vagus x ganglion

strong

homogeneous

strong expression: see section 06 17 18 19 20 moderate expression: see section 07 08

vestibulocochlear viii ganglion

strong

homogeneous

strong expression: see section 19 moderate expression: see section 07 08

cervico-thoracic ganglion

strong

homogeneous

strong expression: see section 10 18

cervical ganglion

strong

homogeneous

strong expression: see section 09 18

peripheral nerve trunk

strong

homogeneous

strong expression: see section 15

dorsal root ganglion

strong

homogeneous

strong expression: see section 08 09 10 11 13 14 15 16 17 18 19 20 21

retina nerve fiber layer

strong

homogeneous

strong expression: see section 01 02 03 24

retina nuclear layer

strong

homogeneous

strong expression: see section 01 02 03 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T1632
Entity Detected:	Smad2, MAD homolog 2 (Drosophila) ( MGI:108051)
Sequence:	sense strand is shown >T1632 AGAATGAGTTTTGTGAAGGGCTGGGGAGCAGAATATCGGAGGCAGACAGTAACAAGTACTCCTTGCTGGA TTGAACTTCATCTGAATGGCCCTCTGCAGTGGCTGGACAAAGTATTAACTCAGATGGGATCCCCTTCAGT GCGATGCTCAAGCATGTCGTAAACCCATCAAAGACTCGCTGTAACAGCTCCTCCGTCGTAGTATTCATGT ATGATCCCGTGGACTGTTTGCTATCCAAAAATTCCAGAGCAAAAACAGCACTTGAGGTCTCATCAGTTAA AGCACCTTGTGGAATCTGTTTCCTATATTTGAATATTAGATGGGAAAATTAGTGTCTAGAAATGCCCTCC CCAGCGGGGAAAAAGAAGACTTAAAGACTTAATGATGTCTTGTTGGGCATAAGACAGTATCCCAAAGGTT ATTAATAACAGTAGTAGTTGTGTACAGGTAATGTGTCCAGACCCAGTATTGCAGTACTATGCTGTTTGTA TACATTCTTAGTTTGCATAAATGAGGTGTGTGTGCTGCTTCT
Notes:	The probe template was PCR amplified from IMAGE:444979 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:444979 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	NMRI
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10008 same embryo
	EMAGE:10007 same embryo
	EurExpress:euxassay_000185 same experiment
	MGI:4828313 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source