EMAGE mouse gene expression spatial database

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EMAGE:10011

Col17a1 collagen, type XVII, alpha 1 ( MGI:88450)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_008156. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_008156_01	euxassay_008156_02	euxassay_008156_03	euxassay_008156_04

euxassay_008156_05	euxassay_008156_06	euxassay_008156_07	euxassay_008156_08	euxassay_008156_09

euxassay_008156_10	euxassay_008156_11	euxassay_008156_12	euxassay_008156_13	euxassay_008156_14

euxassay_008156_15	euxassay_008156_16	euxassay_008156_17	euxassay_008156_18	euxassay_008156_19

euxassay_008156_20	euxassay_008156_21	euxassay_008156_22	euxassay_008156_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10011_wholemount_strong.wlz

10011_wholemount_moderate.wlz

10011_wholemount_weak.wlz

10011_wholemount_possible.wlz

10011_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10011_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

epidermis

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

vibrissa

moderate

regional

moderate expression: see section 19 20 weak expression: see section 01 02 03 17 18

pharyngo-tympanic tube

strong

regional

strong expression: see section 01 02 03 21 22 23 moderate expression: see section 04

cornea epithelium

moderate

regional

moderate expression: see section 01 21 22 23

naris

moderate

regional

moderate expression: see section 06 07 13 weak expression: see section 10 11 12

nasal cavity respiratory epithelium

moderate

regional

moderate expression: see section 07 08 13 weak expression: see section 11 12

esophagus

moderate

regional

moderate expression: see section 13 14 15

oral epithelium

moderate

regional

moderate expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19

larynx

weak

regional

weak expression: see section 13 14

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T171
Entity Detected:	Col17a1, collagen, type XVII, alpha 1 ( MGI:88450)
Sequence:	sense strand is shown >T171 CAGAAGTAACAATTATGACACCACACTAGACCCAGACAGGACAAGAGGGGCAGTGGGCAAGGAAGGGAGA AAAGAGAGGACACAGAACTCCTGATGCTGAGGAAGCATGGCTTCCACACACCATCCTGCACTTAGGAAAT GGCATAAAAGACTGGGCACAGTTGGTTTTTATTTAAGCACTTTCCACTGTCTAAAAACATTTGTATGTAA CCTGTAAGATGAAAAATCATTTCAACTGTAAAATTTTCAATTAAAGTTTTTTTTTCTTTTTTAAAAAAAA AAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:2645686 using vector specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:2645686 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10010 same embryo
	EMAGE:10009 same embryo
	EurExpress:euxassay_008156 same experiment
	MGI:4823981 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source