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EMAGE:10022

Rabac1 Rab acceptor 1 (prenylated) ( MGI:1201692)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:10022 EMAGE:10022 EMAGE:10022 EMAGE:10022 EMAGE:10022
"Pseudo-wholemount" of euxassay_000239. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_000239_01 euxassay_000239_02 euxassay_000239_03 euxassay_000239_04
EMAGE:10022 EMAGE:10022 EMAGE:10022 EMAGE:10022 EMAGE:10022
euxassay_000239_05 euxassay_000239_06 euxassay_000239_07 euxassay_000239_08 euxassay_000239_09
EMAGE:10022 EMAGE:10022 EMAGE:10022 EMAGE:10022 EMAGE:10022
euxassay_000239_10 euxassay_000239_11 euxassay_000239_12 euxassay_000239_13 euxassay_000239_14
EMAGE:10022 EMAGE:10022 EMAGE:10022 EMAGE:10022 EMAGE:10022
euxassay_000239_15 euxassay_000239_16 euxassay_000239_17 euxassay_000239_18 euxassay_000239_19
EMAGE:10022 EMAGE:10022 EMAGE:10022 EMAGE:10022
euxassay_000239_20 euxassay_000239_21 euxassay_000239_22 euxassay_000239_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:10022Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
10022_wholemount_strong.wlz
10022_wholemount_moderate.wlz
10022_wholemount_weak.wlz
10022_wholemount_possible.wlz
10022_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:10022_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
medulla oblongata
weak weak
regionalweak expression: see section 13 14 15 16 17
metencephalon
moderate moderate
regionalmoderate expression: see section 09 10 weak expression: see section 11 12 13 14 15 16 17
facial vii ganglion
moderate moderate
homogeneousmoderate expression: see section 04 05 06 20 21
glossopharyngeal ix ganglion
moderate moderate
homogeneousmoderate expression: see section 05
trigeminal v ganglion
moderate moderate
homogeneousmoderate expression: see section 03 04 05 06 07 08 17 18 19 20 21 22
vagus x ganglion
moderate moderate
homogeneousmoderate expression: see section 08 09 18 19 20
spinal cord
weak weak
regionalweak expression: see section 12 13 15 16 17
spinal component of peripheral nervous system
weak weak
homogeneousweak expression: see section 14
dorsal root ganglion
moderate moderate
homogeneousmoderate expression: see section 09 10 11 12 13 17 18 19
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T272
Entity Detected:Rabac1, Rab acceptor 1 (prenylated) ( MGI:1201692)
Sequence:sense strand is shown

>T272
GTCCCGGCTGGGGTGGGGCCGCCTAGGTGCTGCTGGGCTCCGGGCTATTTACAGCGGTTCTATCCACTGC
AGTGCAGACATGGCGGCCCAGAAGGACCAGCAGAAGGATGCCGAAGGGGAAGGGCTGAGCGCCACGACCC
TGCTGCCCAAGCTGATTCCATCCGGCGCAGGCCGAGAGTGGCTGGAGCGGCGCCNGGGCGAACCATNCCG
GNCCCTGGGGGCCAC
Notes:The probe template was PCR amplified from IMAGE:2655184 using vector specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:2655184 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:10024 same embryo
 EMAGE:10023 same embryo
 EMAGE:10021 same embryo
 EurExpress:euxassay_000239 same experiment
 MGI:4827587 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS