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EMAGE:10027

Slc36a1 solute carrier family 36 (proton/amino acid symporter), member 1 ( MGI:2445299)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:10027 EMAGE:10027 EMAGE:10027 EMAGE:10027 EMAGE:10027
"Pseudo-wholemount" of euxassay_019688. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_019688_01 euxassay_019688_02 euxassay_019688_03 euxassay_019688_04
EMAGE:10027 EMAGE:10027 EMAGE:10027 EMAGE:10027 EMAGE:10027
euxassay_019688_05 euxassay_019688_06 euxassay_019688_07 euxassay_019688_08 euxassay_019688_09
EMAGE:10027 EMAGE:10027 EMAGE:10027 EMAGE:10027 EMAGE:10027
euxassay_019688_10 euxassay_019688_11 euxassay_019688_12 euxassay_019688_13 euxassay_019688_14
EMAGE:10027 EMAGE:10027 EMAGE:10027 EMAGE:10027 EMAGE:10027
euxassay_019688_15 euxassay_019688_16 euxassay_019688_17 euxassay_019688_18 euxassay_019688_19
EMAGE:10027 EMAGE:10027 EMAGE:10027 EMAGE:10027 EMAGE:10027
euxassay_019688_20 euxassay_019688_21 euxassay_019688_22 euxassay_019688_23 euxassay_019688_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:10027Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
10027_wholemount_strong.wlz
10027_wholemount_moderate.wlz
10027_wholemount_weak.wlz
10027_wholemount_possible.wlz
10027_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:10027_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
rib
weak weak
regionalweak expression: see section 05 06 07 08 09 21 22 23
thymus primordium
weak weak
regionalweak expression: see section 12 13 14 15 16 17
pancreas
weak weak
regionalweak expression: see section 09 10 11
thyroid gland
weak weak
regionalweak expression: see section 12 17
medulla oblongata basal plate mantle layer
weak weak
regionalweak expression: see section 09 10 11 17 18
pons mantle layer
weak weak
regionalweak expression: see section 08 19
facial vii ganglion
weak weak
regionalweak expression: see section 05
glossopharyngeal ix ganglion
moderate moderate
regionalmoderate expression: see section 20 21 weak expression: see section 08 09
trigeminal v ganglion
moderate moderate
regionalmoderate expression: see section 21 weak expression: see section 05 06 09 22 23
vagus x ganglion
moderate moderate
regionalmoderate expression: see section 10 19
ventral grey horn
moderate moderate
regionalmoderate expression: see section 17 weak expression: see section 12 13 14 16
cervico-thoracic ganglion
moderate moderate
regionalmoderate expression: see section 11 17 18
cervical ganglion
moderate moderate
regionalmoderate expression: see section 10 18
thoracic ganglion
moderate moderate
regionalmoderate expression: see section 15 weak expression: see section 13 14
rectum
weak weak
regionalweak expression: see section 13
mandible
weak weak
regionalweak expression: see section 04 05 06 07 08 09 17 18 19 20 21 22 23
maxilla
weak weak
regionalweak expression: see section 08 09 17 18 19 21 22
urethra of male
weak weak
regionalweak expression: see section 13 14
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T56009
Entity Detected:Slc36a1, solute carrier family 36 (proton/amino acid symporter), member 1 ( MGI:2445299)
Sequence:sense strand is shown

>T56009
TCGACTCTACATGCCCTCCTTCCTGCCCTTCCTGGTGTTGCTGTCTTTCATCAGGAACCTGCGTGTGTTG
TCCATCTTCTCCCTGCTGGCCAACATCAGCATGTTCGTCAGCCTGATCATGATATACCAGTTCATTGTCC
AGAGGATCCCAGACCCCAGCCACCTCCCCTTGGTGGCTCCATGGAAGACCTACCCTCTGTTCTTTGGCAC
AGCGATTTTTGCTTTTGAAGGCATTGGAGTGGTTCTACCCCTTGAGAACAAAATGAAGGACTCACAGAAA
TTTCCATTGATTCTGTATTTGGGGATGGCTATCATCACTGTACTCTACATCAGCCTGGGGAGCCTGGGGT
ACCTGCAATTTGGAGCTAATATCAAGGGCAGCATCACCCTCAACCTGCCCAACTGCTGGTTGTACCAGTC
GGTGAAGCTGCTGTACTCCATAGGCATCTTCTTCACCTATGCCCTCCAGTTCTATGTCGCAGCTGAGATC
ATCATCCCGGCCATTGTGTCCCGAGTGCCTGAGCATTTCGAGCTGATGGTGGACCTTTGTGTGCGCACCG
CCATGGTCTGTGTGACATGTGTGCTGGCCATCCTCATCCCACGCCTGGACCTGGTCATCTCCCTGGTGGG
CTCTGTGAGCAGCAGCGCCCTGGCCCTCATCATCCCGCCCCTGCTGGAGGTGGTCACCTACTATGGAGAG
GGCATTAGCCCCCTGACCGTCACCAAGGATGCCCTCATCAGCATCCTGGGCTTCGTGGGCTTCGTGGTGG
GGACCTACGAGTCTCTGTGTGAGCTGATCCAGCCAAGCCATAGCGACTCCTCTACCAATTCCACCAGTGC
CTTCATATAAGGATCTGGGTGTGCTCCCTCCACCAGCCTCACCCTGTGTGTCTCCCAGTAGTGTCCCCAC
ATCCTCAGACAGGGTCTCATGTCACCAGAGTTGGCTTTGAACTTGCTTGCTGTGTAGTCAAGGATGACGA
TGGGCTCTCAGTTCTCCTACCGTTGCCTCAAGTATGGAGGTTACTAGCATGAGCTGCCACTCCCAGCTGG
CTCAGAGACCTCAAGCAGATCATGTGAGCATGC
Notes:The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. Forward Primer - name:unspecified, sequence:TCGACTCTACATGCCCTCCT; Reverse Primer - name:unspecified, sequence:GCATGCTCACATGATCTGCT. The reverse primer contains a 5' extension containing an unspecified RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using unspecified polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:MGI:4828208 same experiment
 EurExpress:euxassay_019688 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS