EMAGE mouse gene expression spatial database

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EMAGE:10053

Mrps6 mitochondrial ribosomal protein S6 ( MGI:2153111)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_000228. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_000228_01	euxassay_000228_02	euxassay_000228_03	euxassay_000228_04

euxassay_000228_05	euxassay_000228_06	euxassay_000228_07	euxassay_000228_08	euxassay_000228_09

euxassay_000228_10	euxassay_000228_11	euxassay_000228_12	euxassay_000228_13	euxassay_000228_14

euxassay_000228_15	euxassay_000228_16	euxassay_000228_17	euxassay_000228_18	euxassay_000228_19

euxassay_000228_20

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10053_wholemount_strong.wlz

10053_wholemount_moderate.wlz

10053_wholemount_weak.wlz

10053_wholemount_possible.wlz

10053_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10053_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

medulla oblongata part of 4th ventricle choroid plexus

strong

homogeneous

strong expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

telencephalon

weak

regional

weak expression: see section 10

lateral ventricle choroid plexus

strong

homogeneous

strong expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19

metencephalon

moderate

regional

moderate expression: see section 11 12 13 14 15 16 weak expression: see section 17

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T398
Entity Detected:	Mrps6, mitochondrial ribosomal protein S6 ( MGI:2153111)
Sequence:	sense strand is shown >T398 GTCCGGGAGCTGACCGGCCGTGTTCCTCTCTCGTCTTCCTCTGCGCCCCGCGTCCCCGCCCTCGCGACCC CGGCTCTCCTGGACTCGGCGCCGCCAACCTGGGCGATGCCCCGCTACGAGTTGGCTTTGATTCTGAAAGC CATGCGGCGGCCAGAGACCGCTGCTGCTTTGAAACGTACAATAGAATCCCTGATGGACCGAGGAGCCATA GTGAGGAACTTGGAAAGCCTGGGTGAGCGTGCGCTCCCCTACAGGATCTCGAGTCACAGCCAGCAGCACA GCCGAGGAGGGTATTTCCTGGTGGATTTTTATGCTCCGACAAGTGCTGTGGAGAACATACTGGAACACTT GGCGCGAGACATTGACGTGGTTAGACCAAATATTGTGAAACACCCTCTGACCCAGGAAGTAAAAGAGTGT GACGGCATAGTCCCAGTCCCACTTGAAGAAAAACTGTATTCAACAAAGAGGAGGAAGAAGTGAGAA
Notes:	The probe template was PCR amplified from IMAGE:3156971 using vector specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:3156971 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10054 same embryo
	EMAGE:10052 same embryo
	EurExpress:euxassay_000228 same experiment
	MGI:4826458 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source