EMAGE mouse gene expression spatial database

EMAGE:10089

Srxn1 sulfiredoxin 1 homolog (S. cerevisiae) ( MGI:104971)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_000203. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_000203_01	euxassay_000203_02	euxassay_000203_03	euxassay_000203_04

euxassay_000203_05	euxassay_000203_06	euxassay_000203_07	euxassay_000203_08	euxassay_000203_09

euxassay_000203_10	euxassay_000203_11	euxassay_000203_12	euxassay_000203_13	euxassay_000203_14

euxassay_000203_15	euxassay_000203_16	euxassay_000203_17	euxassay_000203_18	euxassay_000203_19

euxassay_000203_20	euxassay_000203_21	euxassay_000203_22	euxassay_000203_23	euxassay_000203_24

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Expression pattern clarity:
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Expression Pattern Description

Spatial Annotation:

Annotation colour key:

wholemount mapping

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Find spatially similar wholemount expression patterns:

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

adrenal medulla

strong

single cell

strong expression: see section 10 11 12 18 19 20 21

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T328
Entity Detected:	Srxn1, sulfiredoxin 1 homolog (S. cerevisiae) ( MGI:104971)
Sequence:	sense strand is shown >T328 GCCGGGCGGCACTGGCCACCAAGGAAGAGGTATGGGGCTACGCGCGGGTGGAGCGCTCCGGAGGGCGGGT GCGGGGCCCGGGGCGCCCGAGGGACAGGGACCCGGTGGCGCCCAGGGCGGCAGCATCCACTCGGGCTGCA TCGCCACGGTGCACAACGTACCAATCGCCGTGCTCATCCGGCCGCTGCCGTCCGTGCTGGACCCGGCCAA AGTGCAGAGCCTGGTGGACACGATCCTGGCGGACCCTGACAGTGTGCCCCCCATCGACGTCCTCTGGATC AAAGGGGCCCAGGGTGGCGACTACTACTATTCCTTTGGGGGCTGCCACCGCTATGCAGCCTACCAGCAGC TGCAGAGAGAGACCATTCCTGCCAAGCTCGTGAGGTCCACCCTTTCAGACCTGAGGATGTACCTGGGAGC ATCCACACCAGACTTGCAATAGCAACCTCCTGATAGCCCCCTGCCCCT
Notes:	The probe template was PCR amplified from IMAGE:3154456 using vector specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:3154456 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10092 same embryo
	EMAGE:10093 same embryo
	EMAGE:10091 same embryo
	EMAGE:10094 same embryo
	EMAGE:10090 same embryo
	EurExpress:euxassay_000203 same experiment
	MGI:4828451 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source