EMAGE mouse gene expression spatial database

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EMAGE:10105

Pcca propionyl-Coenzyme A carboxylase, alpha polypeptide ( MGI:97499)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_018934. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_018934_01	euxassay_018934_02	euxassay_018934_03	euxassay_018934_04

euxassay_018934_05	euxassay_018934_06	euxassay_018934_07	euxassay_018934_08	euxassay_018934_09

euxassay_018934_10	euxassay_018934_11	euxassay_018934_12	euxassay_018934_13	euxassay_018934_14

euxassay_018934_15	euxassay_018934_16	euxassay_018934_17	euxassay_018934_18	euxassay_018934_19

euxassay_018934_20	euxassay_018934_21	euxassay_018934_22	euxassay_018934_23	euxassay_018934_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10105_wholemount_strong.wlz

10105_wholemount_moderate.wlz

10105_wholemount_weak.wlz

10105_wholemount_possible.wlz

10105_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10105_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

submandibular gland primordium

moderate

regional

moderate expression: see section 07 08 09 10 21 weak expression: see section 17 18 19 20

vibrissa

moderate

regional

moderate expression: see section 05 06 07 21 22 23 weak expression: see section 19 20 24

olfactory cortex ventricular layer

moderate

homogeneous

moderate expression: see section 10 11 16 17

telencephalon ventricular layer

strong

homogeneous

strong expression: see section 03 moderate expression: see section 01 02 04 05 06 07 08 09 10 11 12 15 16 17 18 19 20 21 22 23 24

rest of cerebellum marginal layer

moderate

regional

moderate expression: see section 03 04 05 06 07 13 21 22 23 weak expression: see section 09 10 11 12 14 15 16 17 18 19 20

rest of cerebellum ventricular layer

moderate

regional

moderate expression: see section 04 06 22 weak expression: see section 18 19 20

midbrain ventricular layer

strong

regional

strong expression: see section 11 12 13 14 15 moderate expression: see section 09 10 16 17

nucleus pulposus

moderate

regional

moderate expression: see section 14 15

orbito-sphenoid

moderate

regional

moderate expression: see section 04 05 22 23 weak expression: see section 02 03 21 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T50875
Entity Detected:	Pcca, propionyl-Coenzyme A carboxylase, alpha polypeptide ( MGI:97499)
Sequence:	sense strand is shown >T50875 AAGGAGACATCAGCACTAAGTTTCTCTCTGATGTGTATCCTGATGGCTTCAAAGGGCACACGTTAACACT GAGTGAGAGAAACCAGTTATTGGCCATTGCATCATCTGTATTTGTGGCATCCCAGCTACGAGCTCAGCGC TTCCAAGAACGTTCAAGAGTACCAGTTATTAGGCCTGATGTGGCTAAGTGGGAGCTCTCGGTAAAGTTAC ATGATGAAGATCATACTGTCGTGGCATCTAACAATGGGCCGGCATTTACCGTGGAAGTTGATGGCTCGAA ACTAAATGTGACCAGTACGTGGAACCTGGCGTCACCCTTATTGTCTGTCAACGTTGATGGCACGCAGAGG ACTGTGCAGTGTCTTTCTCGGGAAGCAGGTGGAAACATGAGCATCCAGTTTCTTGGCACAGTGTACAAAG TGCACATTTTAACCAAGCTTGCTGCAGAGCTGAACAAATTCATGCTTGAAAAAGTGCCCAAGGACACCAG CAGCACTCTGTGCTCCCCGATGCCTGGAGTGGTGGTGGCCGTTTCTGTCAAGCCTGGAGACATGGTAGCA GAAGGTCAGGAAATCTGTGTGATTGAAGCTATGAAAATGCAGAACAGTATGACAGCTGGGAAAATGGGCA AGGTGAAATTGGTGCACTGCAAAGCTGGAGACACAGTTGGTGAAGGAGACCTGCTTGTGGAGCTGGAATG AAGTATTTCTAGCCTTTCCGTCAGCACTGCTCACTGCTTTAACTGGCGTCTCCATTATTCTCTAACCCTC AATTTGCGCAAGCATTTACAGGAGCACTCCTGTGCAGGAGGTTCTACAGCCTCCTGTTTATTCCTCAGAA GAGGCAAAACCGACATTCTGCCAACAAATCACCAATGGAAATTCTTGATATAAATACTTGTACATATGAT TTGTACTTCTGCTGAGATTTCCTAGTGTCA
Notes:	The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. Forward Primer - name:unspecified, sequence:AAGGAGACATCAGCACTAAG; Reverse Primer - name:unspecified, sequence:TGACACTAGGAAATCTCAGC. The reverse primer contains a 5' extension containing an unspecified RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using unspecified polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10107 same embryo
	EMAGE:10106 same embryo
	EMAGE:10104 same embryo
	EurExpress:euxassay_018934 same experiment
	MGI:4827076 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source