EMAGE mouse gene expression spatial database

EMAGE:10106

Lrfn3 leucine rich repeat and fibronectin type III domain containing 3 ( MGI:2442512)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_000205. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_000205_01	euxassay_000205_02	euxassay_000205_03	euxassay_000205_04

euxassay_000205_05	euxassay_000205_06	euxassay_000205_07	euxassay_000205_08	euxassay_000205_09

euxassay_000205_10	euxassay_000205_11	euxassay_000205_12	euxassay_000205_13	euxassay_000205_14

euxassay_000205_15	euxassay_000205_16	euxassay_000205_17	euxassay_000205_18	euxassay_000205_19

euxassay_000205_20	euxassay_000205_21	euxassay_000205_22	euxassay_000205_23

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Expression Pattern Description

Spatial Annotation:

Annotation colour key:

wholemount mapping

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Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T1646
Entity Detected:	Lrfn3, leucine rich repeat and fibronectin type III domain containing 3 ( MGI:2442512)
Sequence:	sense strand is shown >T1646 TGGCCTCGAGCCAGATTCGGACGAGGGCGCTGGGAGGTGTCATCGTCGCTTCTGTTCTGGTCTTCATCTT CGTGCTGCTTCTACGTTACAAGGTGCACGGAGGCCAGCCGCCCGGCAAGGCCAAGGCTACGGCACCTGTC AGCAGCGTTTGCTCCCAGACCAACGGAGCGCTCGGGCCCGTGCCCAGTGCGCCTGCCCCTGAGCCTGCTG CACCCAGGGCCCATACTGTGGTCCAATTGGACTGTGAGCCCTGGGGGCCCAGTCACGAACCTGCAGGACC CTAGCCAAGGGCCCACCTCTATGGTTCCTCTGGTCCCAGGGACACCCCTCGGGACCTGGCCTCAGACTCA CCAAATTGCTCACGGTTTTTAAAACTCTGATGGGGAGGGTGTCGGGGGCACCGGGGCACAACAAGAAAGT CCTATTTTTCTAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:458783 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:458783 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10107 same embryo
	EMAGE:10104 same embryo
	EMAGE:10105 same embryo
	EurExpress:euxassay_000205 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source