EMAGE mouse gene expression spatial database

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EMAGE:10109

Cdca2 cell division cycle associated 2 ( MGI:1919787)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_000111. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_000111_01	euxassay_000111_02	euxassay_000111_03	euxassay_000111_04

euxassay_000111_05	euxassay_000111_06	euxassay_000111_07	euxassay_000111_08	euxassay_000111_09

euxassay_000111_10	euxassay_000111_11	euxassay_000111_12	euxassay_000111_13	euxassay_000111_14

euxassay_000111_15	euxassay_000111_16	euxassay_000111_17	euxassay_000111_18	euxassay_000111_19

euxassay_000111_20	euxassay_000111_21	euxassay_000111_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10109_wholemount_strong.wlz

10109_wholemount_moderate.wlz

10109_wholemount_weak.wlz

10109_wholemount_possible.wlz

10109_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10109_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thalamus

weak

homogeneous

weak expression: see section 13

not examined

other

not examined expression: see section 05

cerebral cortex ventricular layer

strong

graded

strong expression: see section 06 07 08 09 10 11 12 14 15 16 17 18 19 20 21 22 moderate expression: see section 02 03 04 05

midbrain

strong

graded

strong expression: see section 16

midbrain ventricular layer

strong

graded

strong expression: see section 10 11 12 14 15 16 17 weak expression: see section 13

metanephros

weak

regional

weak expression: see section 13 14 21 22

renal cortex

weak

regional

weak expression: see section 10 11 18 19 20

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T1515
Entity Detected:	Cdca2, cell division cycle associated 2 ( MGI:1919787)
Sequence:	sense strand is shown >T1515 TCCGTGAGACGCAGCACAAGGCTCCAGAGAGACTTGGAAAATACAGGGCTTGTGTGGCTGTCTCCAAGTC CTTCCACTTTGCAGAAGCCCAGAAGGAGAATGACGATCTGCACCTTAGACAGCAGGGGATTTGAATGTCC ATCCTCCAAAGAAGAGACCATATCCTCAGGACAAAACCCAGGGCCGCTGCCCGCTGTCTCGGGCAGTGAG AGCCAGGGTGCTGGTTCTTCTGCGCTACCTAGGAAGAGGAGAAGCCTTTGTGGGTCTACCCTCACAGATG CTAACAGTGCCACCCAGCCACCAGACTGCAAGAGAAAACCCTCGCTCAAGGGAGAAAGCGCTCAGCTGCC CTGAGGGGGCTGGGCCCAGGAGAACAAAGGCAGGAACAGACAATCCCACCTGGCATTGGCTCTTAGGGAA GAGGACCCAGCTTCCTGTAGGCCGCTTCAGTGCATTGCACATTTCTAATAAAAATCATTTGAGTTATGAT GACTTTAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:761303 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:761303 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10110 same embryo
	EMAGE:10111 same embryo
	EMAGE:10108 same embryo
	EurExpress:euxassay_000111 same experiment
	MGI:4823757 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source