EMAGE mouse gene expression spatial database

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EMAGE:10149

Slc14a1 solute carrier family 14 (urea transporter), member 1 ( MGI:1351654)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_018942. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_018942_01	euxassay_018942_02	euxassay_018942_03	euxassay_018942_04

euxassay_018942_05	euxassay_018942_06	euxassay_018942_07	euxassay_018942_08	euxassay_018942_09

euxassay_018942_10	euxassay_018942_11	euxassay_018942_12	euxassay_018942_13	euxassay_018942_14

euxassay_018942_15	euxassay_018942_16	euxassay_018942_17	euxassay_018942_18	euxassay_018942_19

euxassay_018942_20	euxassay_018942_21	euxassay_018942_22	euxassay_018942_23	euxassay_018942_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10149_wholemount_strong.wlz

10149_wholemount_moderate.wlz

10149_wholemount_weak.wlz

10149_wholemount_possible.wlz

10149_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10149_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

vertebral axis musculature

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 18 19 20 23 24 weak expression: see section 17 21 22

diencephalon lateral wall ventricular layer

weak

regional

weak expression: see section 10 13

telencephalon ventricular layer

weak

regional

weak expression: see section 02 03 04 05 06 07 08 14 15 16 17 18 19 20

medulla oblongata floor plate

strong

regional

strong expression: see section 13 14 15

metencephalon floor plate

strong

regional

strong expression: see section 13

spinal cord floor plate

strong

regional

strong expression: see section 13 14 15 16

eye

moderate

regional

moderate expression: see section 24

extrinsic ocular muscle

moderate

regional

moderate expression: see section 01 02 22 24 weak expression: see section 03 20 21 23

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 06 07 08 weak expression: see section 09 10 13 14 15 16 17

tongue

weak

regional

weak expression: see section 10 11 13 14

liver

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 moderate expression: see section 09

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T51029
Entity Detected:	Slc14a1, solute carrier family 14 (urea transporter), member 1 ( MGI:1351654)
Sequence:	sense strand is shown >T51029 TGGGAGCCACTTCCATACTCTCGGCGATCCATTCTTTTGCTTGCTTCTTGTTTTTCTCTTTGACTTTATA TTATCAATCACATGAGAGCCGTGTCCAGTTGATGTTCTCTGGAATGGAATTTAAACCCCACTGTGCACTG GGGGTCGGCAGGAGTATCACTAAGACTGCATTTAGAGAGTCAAAATGTTCCGACCGACAGAAGGAATGGA AGCAGCAACAATTATTGTTATTTGAAGCTATCTTTGATGTCAGTATTAAAAGTCAAGCTCTGTAAACCAT GTAAGCCTTGCAGAATCCACGGGCACAGAAACAAAGCAGCCCAGGGTTCTCGGAGAGGCCTTTTGCTTTC AGAATCAGTGCAAGTTACTCAGAGCAACCGCATAACAACATTGCAGTGGGGCAGGTTGACCAGAGCGCTA GTCCCCAGCTCTCCAGCTGGGTGGTGATGTTCTCTTAATAAGAAACGTTAGTTAGCTTTTTGTTCCAAGC TGACCGAGCTGTGGGAAGAACTTTGTTGTCCAAAATAGTTTTCCACTGCCACACTCAGAATTTGCAAAAT CTAGTAGAGTTTATTTATTCTTTATGAAAATATTTGCCCACTGATATATGTTTGCAGCTAGCCAGTTCTA AGATATTAAATAAAATGTATAAATGCATAAAATACTAAGCATACTTGCTTTTGCTTTATATACTTGCTTT ACTATATATTAGTTTGGAGGGATTGTTTGACATAGGGCTGTCTATGTAGCCCAGGCTAGCCTAGAACTCA GCATCTCCCTGTTTCCATTCCCTAAGCACCAGAGCTGTGTGTTGGGTTTTTAAGTGCTTATAAGGCAACT TCATCAACCTCGAGAGCCTTTTGAAGCCACAGTTGAATGAATCTGAGGGTATCATCTAAACACCAGCAAC TAAGCTGCAC
Notes:	The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. Forward Primer - name:unspecified, sequence:TGGGAGCCACTTCCATACTC; Reverse Primer - name:unspecified, sequence:GTGCAGCTTAGTTGCTGGTG. The reverse primer contains a 5' extension containing an unspecified RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using unspecified polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10150 same embryo
	EMAGE:10151 same embryo
	EMAGE:10152 same embryo
	EurExpress:euxassay_018942 same experiment
	MGI:4828102 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source