EMAGE mouse gene expression spatial database

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EMAGE:10160

Smc3 structural maintenace of chromosomes 3 ( MGI:1339795)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_000017. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_000017_01	euxassay_000017_02	euxassay_000017_03	euxassay_000017_04

euxassay_000017_05	euxassay_000017_06	euxassay_000017_07	euxassay_000017_08	euxassay_000017_09

euxassay_000017_10	euxassay_000017_11	euxassay_000017_12	euxassay_000017_13	euxassay_000017_14

euxassay_000017_15	euxassay_000017_16	euxassay_000017_17	euxassay_000017_18	euxassay_000017_19

euxassay_000017_20	euxassay_000017_21	euxassay_000017_22	euxassay_000017_23	euxassay_000017_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10160_wholemount_strong.wlz

10160_wholemount_moderate.wlz

10160_wholemount_weak.wlz

10160_wholemount_possible.wlz

10160_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10160_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

upper leg rest of mesenchyme

moderate

spotted

moderate expression: see section 22 23

thymus primordium

moderate

regional

moderate expression: see section 11 12 13 14 16 weak expression: see section 15

submandibular gland primordium

strong

regional

strong expression: see section 06 07 19 20 moderate expression: see section 08 09 17 18

vibrissa

strong

regional

strong expression: see section 17 18 19 moderate expression: see section 03 04 05 06 16 20 weak expression: see section 21

cerebral cortex ventricular layer

moderate

homogeneous

moderate expression: see section 02 03 04 05 06 07 08 09 10 11 15 16 17 18 19 20 21 22 23 24 weak expression: see section 12

dorsal root ganglion

strong

homogeneous

strong expression: see section 18 19 20 21 moderate expression: see section 09 10 11 17 weak expression: see section 16

heart

strong

spotted

strong expression: see section 06 09 10 11 moderate expression: see section 07 12 13 14 15 16

renal cortex

moderate

regional

moderate expression: see section 07 08 17 18 19 weak expression: see section 06 09 20

larynx

strong

regional

strong expression: see section 20

lung

strong

regional

strong expression: see section 04 05 06 08 09 10 11 12 13 14 15 16 17 18 19 21 22 23 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T1795
Entity Detected:	Smc3, structural maintenace of chromosomes 3 ( MGI:1339795)
Sequence:	sense strand is shown >T1795 CAGGAGTTGGGATCAGGGTGTCATTTACAGGGAAGCAAGGGGAGATGAGAGAAATGCAGCAGCTCTCAGG AGGACAGAAATCTCTGGTAGCCCTTGCTCTCATCTTTGCCATTCAGAAATGCGACCCTGCTCCTTTTTAC CTGTTTGATGAGATCGACCAAGCTTTGGATGCTCAGCACAGAAAAGCCGTGTCAGATATGATTATGGAGC TAGCCGTGCATGCCCAGTTTATTACCACTACTTTCAGGCCTGAACTGCTTGAGTCAGCTGACAAATTCTA TGGTGTAAAGTTCAGAAATAAGGTTAGTCACATTGATGTGATCACAGCAGAGATGGCCAAAGATTTTGTA GAAGACGATACCACGCATGGTTAACTGAAGGATAATGCTTGCTGTCTTGGAAGATGTGTATAATAATGTG GTCCATACCCAGAACTGTAAATTTTAAAACTAAATATTTGGTTATTATT
Notes:	The probe template was PCR amplified from IMAGE:574398 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:574398 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10159 same embryo
	EurExpress:euxassay_000017 same experiment
	MGI:4828326 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source