EMAGE mouse gene expression spatial database

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EMAGE:10161

Nhlrc2 NHL repeat containing 2 ( MGI:1914116)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_000151. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_000151_01	euxassay_000151_02	euxassay_000151_03	euxassay_000151_04

euxassay_000151_05	euxassay_000151_06	euxassay_000151_07	euxassay_000151_08	euxassay_000151_09

euxassay_000151_10	euxassay_000151_11	euxassay_000151_12	euxassay_000151_13	euxassay_000151_14

euxassay_000151_15	euxassay_000151_16	euxassay_000151_17	euxassay_000151_18	euxassay_000151_19

euxassay_000151_20	euxassay_000151_21	euxassay_000151_22	euxassay_000151_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10161_wholemount_strong.wlz

10161_wholemount_moderate.wlz

10161_wholemount_weak.wlz

10161_wholemount_possible.wlz

10161_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10161_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

telencephalon ventricular layer

strong

regional

strong expression: see section 05 06 07 09 10 11 12 16 17 18 19 20 21 22 23

trigeminal v ganglion

moderate

homogeneous

moderate expression: see section 09 10 18

vagus x ganglion

moderate

homogeneous

moderate expression: see section 15

inferior vagus x ganglion

moderate

homogeneous

moderate expression: see section 15

oculomotor iii nerve

strong

homogeneous

strong expression: see section 23

olfactory i nerve

strong

homogeneous

strong expression: see section 23

optic ii nerve

strong

homogeneous

strong expression: see section 23

heart valve

strong

regional

strong expression: see section 12 13 14

lower jaw incisor

strong

regional

strong expression: see section 11 12 15

upper jaw incisor

strong

regional

strong expression: see section 12

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T1797
Entity Detected:	Nhlrc2, NHL repeat containing 2 ( MGI:1914116)
Sequence:	sense strand is shown >T1797 TGGCCTCGAGGCCAGATTCGGCACGAGGGTGGAAATGAAGAGAACCGAAACAACGCATATCCTCACAAGG CAGGCTTTGCCCAGCCGTCAGGCCTCGCTCTCGCCTCAGAAGAGCCCTGGAGCTGCCTCTTTGTGGCTGA CAGTGAGAGCAGCACCGTGCGGACCGTCTCGCTGAGAGACGGAGCTGTGAAGCACCTCGTAGGCGGAGAG CGAGACCCCATGAATTTATTTGCTTTTGGTGATGTTGATGGAGCAGGCATCAATGCGAAGCTTCAGCATC CACTTGGGGTTGCATGGGACGAGGAGAGGCAAGTGCTCTACGTGGCCGACTCCTACAATCACAAGATTAA AGTTGTGGATCCAAAAACAAAAGGCTGTACAACGTTAGCAGGGACTGGGGACGCCAGTGATGCCAGTTCC AGTTTTGCGGAGTCAGCCTTTAATGAGCCCGGAGGCTTGTGTATCGGTGAGAGTGGTCGGCTGTTATATG TAGCAGACACTAATAATCACCAAATTAAAGTGATGGAATTTAGAAGCAAGAACAGNTTCTGTGCTCCCAG TCTG
Notes:	The probe template was PCR amplified from IMAGE:574492 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:574492 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10163 same embryo
	EMAGE:10164 same embryo
	EMAGE:10162 same embryo
	EurExpress:euxassay_000151 same experiment
	MGI:4826706 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source