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EMAGE:10162

Nphs2 nephrosis 2 homolog, podocin (human) ( MGI:2157018)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:10162 EMAGE:10162 EMAGE:10162 EMAGE:10162 EMAGE:10162
"Pseudo-wholemount" of euxassay_018938. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_018938_01 euxassay_018938_02 euxassay_018938_03 euxassay_018938_04
EMAGE:10162 EMAGE:10162 EMAGE:10162 EMAGE:10162 EMAGE:10162
euxassay_018938_05 euxassay_018938_06 euxassay_018938_07 euxassay_018938_08 euxassay_018938_09
EMAGE:10162 EMAGE:10162 EMAGE:10162 EMAGE:10162 EMAGE:10162
euxassay_018938_10 euxassay_018938_11 euxassay_018938_12 euxassay_018938_13 euxassay_018938_14
EMAGE:10162 EMAGE:10162 EMAGE:10162 EMAGE:10162 EMAGE:10162
euxassay_018938_15 euxassay_018938_16 euxassay_018938_17 euxassay_018938_18 euxassay_018938_19
EMAGE:10162 EMAGE:10162 EMAGE:10162 EMAGE:10162 EMAGE:10162
euxassay_018938_20 euxassay_018938_21 euxassay_018938_22 euxassay_018938_23 euxassay_018938_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:10162Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
10162_wholemount_strong.wlz
10162_wholemount_moderate.wlz
10162_wholemount_weak.wlz
10162_wholemount_possible.wlz
10162_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:10162_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
liver
strong strong
regionalstrong expression: see section 13 14 15 moderate expression: see section 01 02 05 06 07 08 09 10 11 weak expression: see section 03 04 12 16
metanephros
strong strong
regionalstrong expression: see section 09 10 19 20
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T50719
Entity Detected:Nphs2, nephrosis 2 homolog, podocin (human) ( MGI:2157018)
Sequence:sense strand is shown

>T50719
ACCCACTCATCCAGTTCAGGGCTCCCCTGCCTAGGGAGTGCTGTCACCTATCATGGGCTGGATCTTCGCA
CATTAATTAATTTAAAAACAGTCTCTCTGCAGACATGCCCACAGGATAACCTAATGTAGATAATCCCTCA
TTAGTCTCTCCTGGGGTTATTCTAGGTTGTATCAAGTTGGCAAACAAAACTAACCTTCACATCTACATAG
CAATACCCTGTCTCAAAGCCAACAACAAAAATGGAGTCACTTGTGTCAGTGTTCCAAAAAGTAATTAAAG
CCTAGACCAGTACTTCTCAAGCTTCCTGATGCTGTGACATTTTAACACAGATTCTCACGTTGTGATGACC
CCAACCATAAGGTTATTTTCACTGCTACTTAATAACTGTCATTTTGCAGAATTTTTCATACCAAGTCCTA
ATTGCTTGCTGTCATGTACACTATACTACAATGTATACAAGTATGTGCTAGAGAACTCTCTTACTGGTAA
GAAGTTATGCTGATAATCAACCAGGGATATGTGTCTGGGTCATATTCCTTATGGGATACAGATCTCTTGG
GTAGAAGAGTTACTACCAACAGTAGGGAATGATAGGGCACAGAAAGGACAGATATGGGCACTGTCATACT
CTGTAATACAAGGACACATTTGTACTCAACATTGTTGAAAGGACTCTTCACTGGTAGCTTAGCATACACT
GCTAATGGTTTGTTCTAAGTTAATCACCTTCCATAGCTTAGGTGCTCCTGGATGAGAACACTTTTGATAC
CAACAGAAACTAGAGTTTCCATAAGTATATGGGCCCAAACATCTACACAAAGAGGGGAAAATACAAAGAA
TGATAGGAAGACAGACATCATCACATGTCTCAAAAAAATGTGGCTGATCTCTTGACCAGAGGGGCAAGAC
TGAGTC
Notes:The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. Forward Primer - name:unspecified, sequence:ACCCACTCATCCAGTTCAGG; Reverse Primer - name:unspecified, sequence:GACTCAGTCTTGCCCCTCTG. The reverse primer contains a 5' extension containing an unspecified RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using unspecified polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:10163 same embryo
 EMAGE:10161 same embryo
 EMAGE:10164 same embryo
 EurExpress:euxassay_018938 same experiment
 MGI:4826764 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS