EMAGE mouse gene expression spatial database

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EMAGE:10163

Acyp2 acylphosphatase 2, muscle type ( MGI:1922822)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_000133. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_000133_01	euxassay_000133_02	euxassay_000133_03	euxassay_000133_04

euxassay_000133_05	euxassay_000133_06	euxassay_000133_07	euxassay_000133_08	euxassay_000133_09

euxassay_000133_10	euxassay_000133_11	euxassay_000133_12	euxassay_000133_13	euxassay_000133_14

euxassay_000133_15	euxassay_000133_16	euxassay_000133_17	euxassay_000133_18	euxassay_000133_19

euxassay_000133_20	euxassay_000133_21	euxassay_000133_22	euxassay_000133_23	euxassay_000133_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

EMAGE:10163

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10163_wholemount_strong.wlz

10163_wholemount_moderate.wlz

10163_wholemount_weak.wlz

10163_wholemount_possible.wlz

10163_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10163_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

two stars

Text Annotation:

Structure

Level

Pattern

Notes

mylohyoid

moderate

homogeneous

moderate expression: see section 19

pectoralis major

moderate

homogeneous

moderate expression: see section 19

pectoralis minor

moderate

homogeneous

moderate expression: see section 19

sublingual gland primordium

moderate

homogeneous

moderate expression: see section 19

submandibular gland primordium

moderate

homogeneous

moderate expression: see section 19

trigeminal v ganglion

moderate

homogeneous

moderate expression: see section 19

vagus x ganglion

moderate

homogeneous

moderate expression: see section 19

nucleus pulposus

moderate

homogeneous

moderate expression: see section 13

viscerocranium

moderate

homogeneous

Expression in the turbinate bone.

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T1801
Entity Detected:	Acyp2, acylphosphatase 2, muscle type ( MGI:1922822)
Sequence:	sense strand is shown >T1801 CTGCTCGGGAGAAGGCTGGCCGCCATGACCGAGCTGCTCAAGTCTGTGGACTACGAAGTGTTCGGGACAG TGCAAGGTGTTTGCTTCAGAATGTACACAGAAGGAGAAGCTAAGAAACGAGGTCTGGTTGGCTGGGTGAA GAACACAAGCAAAGGCACAGTGACAGGTCAAGTTCAAGGCCCCGAAGAAAAGGTTGATGCCATGAAGTCC TGGCTGAGCAAGGTTGGGAGTCCTAGTTCTCGCATCGACCGGGCCGACTTTTCTAACGAAAAAACAATCT CTAAATTGGAATACTCTGATTTTAGTATTCGATATTAATGGAAGAAAAATATCAATTGCACTGCTTATGT TATAGAAAATAAAACATTCTTAGATTCATAATAGTAGGAGAGTTAAAAATGGAAAGTGTCTCTTCTGGAA TTATACATTACTGAAATGTCTGAAACTGAAAACATTTACTCACCTATGTTTTAGCAAGCAGAAACTAAAT ATTATAAAATAAAATGTCATTATAAATTAT
Notes:	The probe template was PCR amplified from IMAGE:575063 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:575063 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10161 same embryo
	EMAGE:10164 same embryo
	EMAGE:10162 same embryo
	EurExpress:euxassay_000133 same experiment
	MGI:4822968 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source