EMAGE mouse gene expression spatial database

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EMAGE:10178

Slc22a4 solute carrier family 22 (organic cation transporter), member 4 ( MGI:1353479)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_019709. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_019709_01	euxassay_019709_02	euxassay_019709_03	euxassay_019709_04

euxassay_019709_05	euxassay_019709_06	euxassay_019709_07	euxassay_019709_08	euxassay_019709_09

euxassay_019709_10	euxassay_019709_11	euxassay_019709_12	euxassay_019709_13	euxassay_019709_14

euxassay_019709_15	euxassay_019709_16	euxassay_019709_17	euxassay_019709_18	euxassay_019709_19

euxassay_019709_20	euxassay_019709_21	euxassay_019709_22	euxassay_019709_23	euxassay_019709_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10178_wholemount_strong.wlz

10178_wholemount_moderate.wlz

10178_wholemount_weak.wlz

10178_wholemount_possible.wlz

10178_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10178_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

forebrain

moderate

regional

moderate expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

hindbrain

moderate

regional

moderate expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

midbrain

moderate

regional

moderate expression: see section 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21

facial vii ganglion

strong

regional

strong expression: see section 05 06 07 22 23 24

glossopharyngeal ix ganglion

strong

regional

strong expression: see section 07 08 20 21

trigeminal v ganglion

strong

regional

strong expression: see section 04 05 06 07 08 09 10 11 20 21 22 23 24

vagus x ganglion

strong

regional

strong expression: see section 09 19

vestibulocochlear viii ganglion

strong

regional

strong expression: see section 07 08 09 19 20 21

trigeminal v nerve

strong

regional

strong expression: see section 12 20

spinal cord

moderate

regional

moderate expression: see section 09 10 11 12 13 14 15 16

cervico-thoracic ganglion

moderate

regional

moderate expression: see section 10 17

cervical ganglion

moderate

regional

moderate expression: see section 10 18

thoracic ganglion

moderate

regional

moderate expression: see section 12 13 14

dorsal root ganglion

strong

regional

strong expression: see section 07 08 09 10 11 12 16 17 18 19

neural retina

strong

regional

strong expression: see section 02 03 04 05

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 12 13 14 15 16 17 18 19 20 21 weak expression: see section 11 22

vomeronasal organ

moderate

regional

moderate expression: see section 15 18

liver lobe

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

axial skeleton

weak

regional

weak expression: see section 12 13 14 15

axial skeleton tail region

moderate

regional

moderate expression: see section 16 17 18 weak expression: see section 19 20

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T56030
Entity Detected:	Slc22a4, solute carrier family 22 (organic cation transporter), member 4 ( MGI:1353479)
Sequence:	sense strand is shown >T56030 CCAGAGGAGATTTGCAGAGGCCGAACAGATCATCCAGAAAGCCGCAAAGATGAACAGCATCGTGGCGCCA GCAGGGATATTCGATCCTCTAGAGCTACAGGAGCTAAACTCCTTGAAGCAGCAGAAAGTCATAATCCTGG ACCTGTTCAGGACTCGGAACATTGCCACCATAACCGTGATGGCTGTGATGCTGTGGATGCTAACCTCAGT GGGTTACTTTGCTCTGTCTCTCAATGTTCCTAATTTACATGGAGATGTCTACCTGAACTGCTTCCTCTCT GGCCTGATTGAAGTTCCAGCTTACTTCACAGCCTGGCTGCTACTGCGAACCCTGCCACGGAGATATATTA TAGCTGGGGTGCTATTCTGGGGAGGAGGTGTGCTTCTCTTGATCCAAGTGGTACCTGAAGATTATAACTT TGTGTCCATTGGACTGGTGATGCTGGGGAAATTTGGGATCACCTCTGCCTTCTCCATGTTGTATGTCTTC ACTGCGGAGCTCTACCCAACCCTGGTCAGGAACATGGCTGTGGGCATCACCTCCATGGCCTCTCGGGTGG GCAGCATCATTGCCCCCTATTTCGTTTACCTGGGCGCCTATAACAGACTCCTACCCTACATCCTCATGGG CAGTCTGACTGTCCTCATTGGAATCATCACGCTTTTTTTCCCTGAAAGTTTTGGAGTGACTCTACCAGAG AACTTGGAGCAGATGCAGAAAGTGAGAGGGTTCAGATGTGGGAAAAAATCAACAGTCTCAGTGGACAGAG AAGAAAGCCCCAAGGTTCTAATAACTGCATTCTAACGAGGTTTCCAAGGCACTTGGCAAACTGAAAAGCA GATGTATACAATGAGCAGGGTGTGATAGAGCAAGCCTGCAATCCCAGCGCTCTTGGGGTGGAGACAGAAG ATCAGGAGTTCAAGGTCATCCTTGGCTACAGCAGGAGTGTAAGACCAGCCTGTCTTACCACAAGCAACCC TGTCTCAACA
Notes:	The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. Forward Primer - name:unspecified, sequence:CCAGAGGAGATTTGCAGAGG; Reverse Primer - name:unspecified, sequence:TGTTGAGACAGGGTTGCTTG. The reverse primer contains a 5' extension containing an unspecified RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using unspecified polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10177 same embryo
	EurExpress:euxassay_019709 same experiment
	MGI:4828137 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source