Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:10192

Rspo4 R-spondin family, member 4 ( MGI:1924467)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:10192 EMAGE:10192 EMAGE:10192 EMAGE:10192 EMAGE:10192
"Pseudo-wholemount" of euxassay_018898. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_018898_01 euxassay_018898_02 euxassay_018898_03 euxassay_018898_04
EMAGE:10192 EMAGE:10192 EMAGE:10192 EMAGE:10192 EMAGE:10192
euxassay_018898_05 euxassay_018898_06 euxassay_018898_07 euxassay_018898_08 euxassay_018898_09
EMAGE:10192 EMAGE:10192 EMAGE:10192 EMAGE:10192 EMAGE:10192
euxassay_018898_10 euxassay_018898_11 euxassay_018898_12 euxassay_018898_13 euxassay_018898_14
EMAGE:10192 EMAGE:10192 EMAGE:10192 EMAGE:10192 EMAGE:10192
euxassay_018898_15 euxassay_018898_16 euxassay_018898_17 euxassay_018898_18 euxassay_018898_19
EMAGE:10192 EMAGE:10192 EMAGE:10192
euxassay_018898_20 euxassay_018898_21 euxassay_018898_22

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:10192Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
10192_wholemount_strong.wlz
10192_wholemount_moderate.wlz
10192_wholemount_weak.wlz
10192_wholemount_possible.wlz
10192_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:10192_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T50974
Entity Detected:Rspo4, R-spondin family, member 4 ( MGI:1924467)
Sequence:sense strand is shown

>T50974
TACCGAAGGAAGAAGCAAGCAGGCACTGGTCTGGGGGGCAACTGCACGGGCTGTGTCATATGCTCGGAGG
AGAATGGCTGTTCCACCTGCCAGCAGAGGCTCTTCCTCTTCATCCGCCGTGAAGGCATCCGTCAGTACGG
CAAGTGTGTGCACGACTGTCCCCTTGGCTTCTTTGGGATCCGTGGCCAGGAGGCCAACAGGTGCAAGAAA
TGTGGGGCCACGTGCGAGAGCTGTTTCAGCCAGGACTTCTGTATCCGCTGCAAAAGGCGGTTTCACCTAT
ACAAAGGGAAGTGCCTGCCCTCCTGTCCAACCGGCACCTTGACCACCAGAGTACCGGGAGTGCCAGGAGG
AGTGTGAGCCGAGCCCTTGGGGCAGGTGAAGTCCCGGCAAACACAATGGGAAGACCTGTGGCTCAGGCTG
GGGCCTAGAAACCCGGGTCCGGGAGGATGGCCCGCCCAAGCAGGAAGAGACGGCGAGTTGCCGAGTGTTA
TCTGAGTCCAGGAAGTGTCCTATAAAGAGACTCTGCCCAGGAGAAAGAAATCCCAGGCAGAAGAACAGGA
AGGATCGGCGCCAGCGTAAGGACAGGAAGTTGGAGCGCAGGCCACACCAGCGTGGCTCACAGTGACCATC
TCCAGCTCTGGCCTATGCCTTCCGCTCGCCGCACTTCCACAGTGGGCTCCTCGCTTCTCCCCTGTCCAGC
TCTTTCTCCACATTCTTCACTCTTTCTACCTTTCCCGTCTGTCTGCTCTTTCCTTTAGATTTTATCCGTC
TCCCTTTTCCTCTTGCCCTCACCTTCTTCTTCCTGTCCTTCTTTGAAACTTTCCTTCTCCCCGTGTACCC
TTCTCTTCATTCCCTTCTGTTTTCCAAAAAGATCTTCACCGTCCACCCCGCACACGGAGACAGACATAAG
AGAGAGATTAAGCTTGTATCCTCCATGTGGC
Notes:The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. Forward Primer - name:unspecified, sequence:TACCGAAGGAAGAAGCAAGC; Reverse Primer - name:unspecified, sequence:GCCACATGGAGGATACAAGC. The reverse primer contains a 5' extension containing an unspecified RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using unspecified polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:10191 same embryo
 EMAGE:10193 same embryo
 EurExpress:euxassay_018898 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS