EMAGE mouse gene expression spatial database

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EMAGE:10196

Atp6v1b1 ATPase, H+ transporting, lysosomal V1 subunit B1 ( MGI:103285)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_000004. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_000004_01	euxassay_000004_02	euxassay_000004_03	euxassay_000004_04

euxassay_000004_05	euxassay_000004_06	euxassay_000004_07	euxassay_000004_08	euxassay_000004_09

euxassay_000004_10	euxassay_000004_11	euxassay_000004_12	euxassay_000004_13	euxassay_000004_14

euxassay_000004_15	euxassay_000004_16	euxassay_000004_17	euxassay_000004_18	euxassay_000004_19

euxassay_000004_20	euxassay_000004_21	euxassay_000004_22	euxassay_000004_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10196_wholemount_strong.wlz

10196_wholemount_moderate.wlz

10196_wholemount_weak.wlz

10196_wholemount_possible.wlz

10196_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10196_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

embryo

strong

regional

strong expression: see section 18

diaphragm

weak

regional

weak expression: see section 04 21

pericardial cavity

moderate

regional

moderate expression: see section 22 weak expression: see section 04

limb

strong

regional

strong expression: see section 09 21 moderate expression: see section 01 02 04 05 23

forelimb

moderate

regional

moderate expression: see section 03 04 22

arm

moderate

regional

moderate expression: see section 03

elbow mesenchyme

moderate

regional

moderate expression: see section 03 04

forearm mesenchyme

moderate

regional

moderate expression: see section 03 04

shoulder mesenchyme

moderate

regional

moderate expression: see section 03 04

hand

moderate

homogeneous

moderate expression: see section 22

hand mesenchyme

moderate

regional

moderate expression: see section 04

hindlimb

strong

regional

strong expression: see section 09 21 moderate expression: see section 03 06 08 23

foot

moderate

regional

moderate expression: see section 22 23

foot mesenchyme

strong

regional

strong expression: see section 09

foot skin

strong

regional

strong expression: see section 09

hip mesenchyme

moderate

regional

moderate expression: see section 04

lower leg mesenchyme

moderate

regional

moderate expression: see section 04

upper leg mesenchyme

moderate

regional

moderate expression: see section 04

axial musculature

weak

regional

weak expression: see section 04

salivary gland

strong

homogeneous

strong expression: see section 19

sublingual gland primordium

strong

regional

strong expression: see section 13 19 21 moderate expression: see section 12 14

submandibular gland primordium

strong

regional

strong expression: see section 09 10 11 12 19 20 21

brain

strong

regional

strong expression: see section 08

physiological umbilical hernia

strong

regional

strong expression: see section 17 18 20 21 moderate expression: see section 15 16 19

physiological umbilical hernia dermis

strong

regional

strong expression: see section 17 18 20 moderate expression: see section 14 15 16 19

vibrissa

strong

regional

strong expression: see section 06 08 09 10 11 12 13 23 moderate expression: see section 05 21 22

vibrissa dermal component

strong

regional

strong expression: see section 06 08 09 10 11 23 moderate expression: see section 04 21 22

vibrissa epidermal component

strong

regional

strong expression: see section 11 12 13 23

telencephalon

strong

regional

strong expression: see section 04 08 10 11 21 moderate expression: see section 15

cerebral cortex

strong

regional

strong expression: see section 03 04 05 06 08 09 11 12 14 16 17 21 22 23 moderate expression: see section 13 15

cerebral cortex mantle layer

strong

homogeneous

strong expression: see section 16

cerebral cortex marginal layer

strong

regional

strong expression: see section 04 05 06 08 09 10 12 14 16 17 20 21 22 23 moderate expression: see section 13 15

cerebral cortex ventricular layer

strong

regional

strong expression: see section 03 04 05 06 08 09 10 11 12 17 20 21 22 23 moderate expression: see section 13

lateral ventricle

strong

graded

strong expression: see section 12

olfactory lobe

strong

graded

strong expression: see section 12 14 16

olfactory cortex

moderate

graded

moderate expression: see section 13

olfactory cortex mantle layer

strong

regional

strong expression: see section 14 16

olfactory cortex marginal layer

strong

graded

strong expression: see section 14 16 moderate expression: see section 13

medulla oblongata alar plate ventricular layer

moderate

regional

moderate expression: see section 14

medulla oblongata sulcus limitans

strong

homogeneous

strong expression: see section 14

rest of cerebellum marginal layer

strong

regional

strong expression: see section 14

pons

strong

regional

strong expression: see section 14 moderate expression: see section 11 13 weak expression: see section 12 15 17

pons marginal layer

moderate

regional

moderate expression: see section 11

midbrain

moderate

graded

moderate expression: see section 12 13

midbrain lateral wall

moderate

graded

moderate expression: see section 12 16

midbrain mantle layer

strong

graded

strong expression: see section 14 moderate expression: see section 13 15 16 weak expression: see section 17

midbrain marginal layer

strong

graded

strong expression: see section 14 moderate expression: see section 12 13 15 16

midbrain ventricular layer

strong

graded

strong expression: see section 14 moderate expression: see section 12 13

trigeminal v ganglion

strong

regional

strong expression: see section 05 06 08 09 10 11 17 19 20 21 22 moderate expression: see section 23

vagus x ganglion

weak

regional

weak expression: see section 15

superior vagus x ganglion

weak

graded

weak expression: see section 14

central nervous system nerve

weak

graded

weak expression: see section 14

hypoglossal xii nerve

weak

graded

weak expression: see section 14 15

trigeminal v nerve

strong

regional

strong expression: see section 08 09 10 11 17 19

autonomic ganglion

strong

homogeneous

strong expression: see section 17

spinal component of peripheral nervous system

strong

regional

strong expression: see section 18 20 21

spinal ganglion

strong

regional

strong expression: see section 18 20 21

dorsal root ganglion

strong

regional

strong expression: see section 10 12 13 14 18 19 20 21 moderate expression: see section 09 16 17

cornea

strong

regional

strong expression: see section 04

cornea stroma

strong

regional

strong expression: see section 03 04 weak expression: see section 01 02

neural retina

weak

regional

weak expression: see section 01 02 03 04

nose

strong

regional

strong expression: see section 19

naris

strong

regional

strong expression: see section 14 23

external naris

strong

regional

strong expression: see section 23

nasal capsule

strong

regional

strong expression: see section 14

nasal cavity

strong

regional

strong expression: see section 13 14 moderate expression: see section 12 21

nasal cavity epithelium

strong

regional

strong expression: see section 14

nasal cavity olfactory epithelium

strong

regional

strong expression: see section 17

nasal cavity respiratory epithelium

strong

regional

strong expression: see section 17 moderate expression: see section 12

nasal septum

strong

regional

strong expression: see section 17

viscerocranium

strong

regional

Expression in the turbinate bone.

lower jaw

moderate

regional

moderate expression: see section 16

oral epithelium

moderate

regional

moderate expression: see section 16

lower lip

moderate

graded

moderate expression: see section 13

lower jaw skeleton

strong

regional

strong expression: see section 20

lower jaw mesenchyme

strong

regional

strong expression: see section 10 moderate expression: see section 14 16

lower jaw tooth

moderate

regional

moderate expression: see section 17

not examined

other

not examined expression: see section 12

upper lip

moderate

graded

moderate expression: see section 13

liver and biliary system

moderate

regional

moderate expression: see section 06

not examined

other

not examined expression: see section 12

urinary system

weak

regional

weak expression: see section 18

metanephros

moderate

regional

moderate expression: see section 11 20 21 22 weak expression: see section 12 18 19

metanephros

moderate

spotted

moderate expression: see section 10

reproductive system

moderate

regional

moderate expression: see section 23 weak expression: see section 08 09

male reproductive system

moderate

regional

moderate expression: see section 23 weak expression: see section 08 09

testis

moderate

regional

moderate expression: see section 23 weak expression: see section 08

lung

strong

regional

strong expression: see section 09 12 18 19 21 23 moderate expression: see section 13 15 22

left lung

strong

regional

strong expression: see section 17 18 19 20 21 23 moderate expression: see section 22

right lung

strong

regional

strong expression: see section 09 10 11 moderate expression: see section 06 08 15

right lung accessory lobe

strong

regional

strong expression: see section 09 10 11 moderate expression: see section 06 08

right lung accessory lobe lobar bronchus

moderate

regional

moderate expression: see section 06

nucleus pulposus

strong

spotted

strong expression: see section 15 16

jugular foramen

strong

regional

strong expression: see section 09

sphenoid bone

strong

regional

strong expression: see section 10 11 12

ethmoid bone primordium

strong

regional

strong expression: see section 10 11 12 moderate expression: see section 04 weak expression: see section 03

facial bone primordium

moderate

regional

moderate expression: see section 04 weak expression: see section 03

clavicle

moderate

regional

moderate expression: see section 12

tail ganglion

strong

homogeneous

strong expression: see section 13

tail dorsal root ganglion

strong

homogeneous

strong expression: see section 13 14 moderate expression: see section 15

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T1472
Entity Detected:	Atp6v1b1, ATPase, H+ transporting, lysosomal V1 subunit B1 ( MGI:103285)
Sequence:	sense strand is shown >T1472 CCTCNANNCTGTTGGCCTACTGGAGATAAAACTGAAGTCTTAGAGCTACCACCAGGCTTCTCAGGCACTA GCTCCCAGCTCTCCACACGCCATGGCCACAACAGTAGACAGCAGGTCCTCGGGATTCACTGGCAATAGCT GTGACCCAGGCACAGCCCAAGAGCATGTGCAGGCTGTGACCCGAAACTACATCACCCACCCCCGAGTCAC CTATAGGACAGTGTGCAGCGTCAATGGGCCTCTGGTGGTGCTGGACCAGGTCAAGTTTGCCCAGTATGCT GAGATTGTCAACTTTACCCTCCCTGATGGTACTCAGAGGAGTGGCCAGGTGCTTGAAGTGGCTGGGACCA AGGCCATTGTTCAGGTGTTTGAAGGGACTTCAGGGATTGATTCCCAGAAGACCACATGTGAATTCACAGG GGACATCCTGCGGACTCCTGTGTCAGAAGACATGCTGGGTCGGATTTTCAATGGCTCTGGCAAACCCATT GACAAAGGACCTGCTGTCATGGCAGAGGAGTTTCTGGACATCAATGGCCAGCCAATCAATCCCCATGACC GAATCTACCCCGAGGAGATGA
Notes:	The probe template was PCR amplified from IMAGE:2537004 using vector specific primers. Forward Primer - name:T7-pME18S-FL3-fw, sequence:CGTAATACGACTCACTATAGGGCCTTCTGCTCTAAAAGCTGCG; Reverse Primer - name:T3-pME18S-FL3-rv, sequence:CAAATTAACCCTCACTAAAGGGCGACCTGCAGCTCGAGCAC. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:2537004 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10202 same embryo
	EMAGE:10200 same embryo
	EMAGE:10198 same embryo
	EMAGE:10199 same embryo
	EMAGE:10201 same embryo
	EMAGE:10197 same embryo
	EurExpress:euxassay_000004 same experiment
	MGI:4823327 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source