EMAGE mouse gene expression spatial database

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EMAGE:10206

Slc25a15 solute carrier family 25 (mitochondrial carrier ornithine transporter), member 15 ( MGI:1342274)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_019703. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_019703_01	euxassay_019703_02	euxassay_019703_03	euxassay_019703_04

euxassay_019703_05	euxassay_019703_06	euxassay_019703_07	euxassay_019703_08	euxassay_019703_09

euxassay_019703_10	euxassay_019703_11	euxassay_019703_12	euxassay_019703_13	euxassay_019703_14

euxassay_019703_15	euxassay_019703_16	euxassay_019703_17	euxassay_019703_18	euxassay_019703_19

euxassay_019703_20	euxassay_019703_21	euxassay_019703_22	euxassay_019703_23	euxassay_019703_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10206_wholemount_strong.wlz

10206_wholemount_moderate.wlz

10206_wholemount_weak.wlz

10206_wholemount_possible.wlz

10206_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10206_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

midgut

moderate

regional

moderate expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

liver

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 13 17 18 19 20 21 22 23 24 weak expression: see section 08 09 10 11 12 14 15 16

metanephros

weak

regional

weak expression: see section 08 09 10 20 21 22

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T56024
Entity Detected:	Slc25a15, solute carrier family 25 (mitochondrial carrier ornithine transporter), member 15 ( MGI:1342274)
Sequence:	sense strand is shown >T56024 TCCTGAAAGACAGGGACTGCCCTGCCCTGCTCTCTTGAAAACCAACAGAGGTTCTTGCCTGCTCTCAGCA GGAATCCAGGGTGCACCTTTGGTACTGGATCTGAGGCCACTCTGAGCCCACTGTGTCTCACCAATCAGAC GCCCTTTCAGGAAGCAGAAAGGAAGTCTGGGCTCCCCGCTTTGATCACAGGGTTCTACTAATGGAGACAT CCGAAGGAGCCCGTTAACTGGGTACCCTTTCTGAAGCTCACTTTTGGGTCCATGATTCCTTGATCTGCTT CTGTATTTGGAGCTGGCAGTTTATGAATTTCAATGACTGTAGATGATATATAACTTTGGAAAGCATTGTG ACATAATAAAGGTATTTTTATTAAATTAGGGTAGTATGTATGTAGCAACCAAAAAACTGGACCCCACATT TTTCTTCTTTGGCTGTGGGGTCTTCTCGCCATAGAGATCAGGCACATTGTCTGTACTGCTCCAAGACCAG GGGGATGGTAGTGGGCGCTGTTCCCACTCTGCCTGTAAGGCTGAAGGAGGTAGGTTGACCTTACCACAGT GTCAGGAGTACAAGGGTCAGGAAAGCAAAGTATTTCCAAAAGCGGGGTGGGGAGGGACACCCGTCAGCAT TCCTGGAGAAAATTCGGCATTCATGAGAAAACTGTCTTTCTACTCCAGTGCTCTCAGTGACCAATGGCTA CTGAGTGCTGCTTCATCTGAACTGATCTGAATTGAGGCAAATGTAGGGTTGGCTTCCTGCAGGAAGACTG TTCAAAGCCAAGCTCTTTCCCTTCTAGGTGCCTGGGTCTAGTGCACATTAGTCTTGTTGGCAGCGTGTCT CCTCAGTCTGGCTATTGTGATCTTTCCCATAGAAAGAGTCAGGAACGAGGGGAAGGGAAAGATAGAGGCC TAAGGTGAAATTTTAAAAAACTCAATCTGTTGGTTTGATTTGTGGTTTCATGTTTGGGTGCAATTGT
Notes:	The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. Forward Primer - name:unspecified, sequence:TCCTGAAAGACAGGGACTGC; Reverse Primer - name:unspecified, sequence:ACAATTGCACCCAAACATGA. The reverse primer contains a 5' extension containing an unspecified RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using unspecified polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	MGI:4828149 same experiment
	EurExpress:euxassay_019703 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source