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EMAGE:1022

Pax3 paired box gene 3 ( MGI:97487)
TS16 (10.0 dpc)
in situ hybridisation

Data Images
EMAGE:1022 EMAGE:1022
Figure 1A of Dietrich et al., 1999 [PMID:10079225] Copyright: This image is from Development and is displayed with the permission of The Company of Biologists Ltd. who owns the copyright. Figure 1B of Dietrich et al., 1999 [PMID:10079225] Copyright: This image is from Development and is displayed with the permission of The Company of Biologists Ltd. who owns the copyright.

Expression pattern clarity: one star
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Figure B is a higher magnification image of part of figure A. Image annotations: arrow indicates the up-regulated Pax3 expression domain in the lateral dermomyotome; arrowheads indicate occipital and cervical somites where migratory hypaxial precursors delaminate from the lateral dermomyotome.
Expression Pattern Description
Spatial Annotation:
EMAGE:1022Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
1022_wholemount_strong_3D_1.wlz
1022_wholemount_moderate_3D_1.wlz
1022_wholemount_weak_3D_1.wlz
1022_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:1022_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
trunk somite
detected detected
regionalExpression is upregulated in the lateral dermomyotome.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:1101193
Entity Detected:Pax3, paired box gene 3 ( MGI:97487)
Notes:The Pax3 probe used in this study by Dietrich et al., 1999 [PMID:10079225] is described as "500 bp encoding the C-terminal part of the protein that includes the homeobox sequence (Goulding et al., 1991 [PMID:2022185] )."
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:129Ola/C57BL6
Age:10.0 dpc
Theiler Stage:TS16
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
Staining procedure:alkaline phosphatase + unspecified
General Information
Authors:Dietrich et al., 1999 [PMID:10079225] Indexed by GXD, Spatially Mapped by EMAGE
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:10079225] Dietrich S, Abou-Rebyeh F, Brohmann H, Bladt F, Sonnenberg-Riethmacher E, Yamaai T, Lumsden A, Brand-Saberi B, Birchmeier C 1999 The role of SF/HGF and c-Met in the development of skeletal muscle. Development (126):1621-9
 [ PMID:2022185] Goulding MD, Chalepakis G, Deutsch U, Erselius JR, Gruss P 1991 Pax-3, a novel murine DNA binding protein expressed during early neurogenesis. EMBO J (10):1135-47
Links:MGI:1333176 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI