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EMAGE:10222

Gstm7 glutathione S-transferase, mu 7 ( MGI:1915562)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:10222 EMAGE:10222 EMAGE:10222 EMAGE:10222 EMAGE:10222
"Pseudo-wholemount" of euxassay_018910. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_018910_01 euxassay_018910_02 euxassay_018910_03 euxassay_018910_04
EMAGE:10222 EMAGE:10222 EMAGE:10222 EMAGE:10222 EMAGE:10222
euxassay_018910_05 euxassay_018910_06 euxassay_018910_07 euxassay_018910_08 euxassay_018910_09
EMAGE:10222 EMAGE:10222 EMAGE:10222 EMAGE:10222 EMAGE:10222
euxassay_018910_10 euxassay_018910_11 euxassay_018910_12 euxassay_018910_13 euxassay_018910_14
EMAGE:10222 EMAGE:10222 EMAGE:10222 EMAGE:10222 EMAGE:10222
euxassay_018910_15 euxassay_018910_16 euxassay_018910_17 euxassay_018910_18 euxassay_018910_19
EMAGE:10222 EMAGE:10222 EMAGE:10222 EMAGE:10222 EMAGE:10222
euxassay_018910_20 euxassay_018910_21 euxassay_018910_22 euxassay_018910_23 euxassay_018910_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:10222Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
10222_wholemount_strong.wlz
10222_wholemount_moderate.wlz
10222_wholemount_weak.wlz
10222_wholemount_possible.wlz
10222_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:10222_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
thymus primordium
moderate moderate
regionalmoderate expression: see section 19 weak expression: see section 15 16 18
pancreas
moderate moderate
regionalmoderate expression: see section 11 12 13 14 15 16 19 20
nasal cavity olfactory epithelium
strong strong
regionalstrong expression: see section 08 09 10 11 12 13 15 16 17 18 19 20 21
stomach
strong strong
regionalstrong expression: see section 11 12 13 moderate expression: see section 07 08 09 10
liver
moderate moderate
regionalmoderate expression: see section 09 10 21 22 23 weak expression: see section 01 02 03 04 05 06 07 08 11 12 13 14 15 16 17 18 19 20 24
urinary system
strong strong
regionalstrong expression: see section 07 08 09
testis
strong strong
regionalstrong expression: see section 07 08 09 10 11 24
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T50468
Entity Detected:Gstm7, glutathione S-transferase, mu 7 ( MGI:1915562)
Sequence:sense strand is shown

>T50468
ATCCGTTTCTGGCAGCTCTCTGAACAGCCAAGGCTAGATTGACACTGAGCCTGTCCGTTCAGACCTCGGC
ACCATGCCCATGACACTGGGTTACTGGGACATCCGTGGGCTAGCCCATGCCATTCGCCTGTTCCTGGAAT
ACACAGACTCAAGCTATGAGGAGAAGAGATACACCATGGGTGATGCTCCTGACTATGACCAAAGCCAGTG
GCTGAATGAGAAATTCAAGCTGGGCCTGGACTTTCCTAACCTGCCCTACTTGATCGATGGGTCACACAAG
ATCACGCAGAGCAATGCCATCCTGCGCTACCTTGGCCGCAAGCACAACCTGTGTGGGGAGACAGAGGAGG
AAAGGATCCGTGTGGATATTCTGGAGAATCAGCTTATGGACAACCGCATGGTGCTGGCGAGGCTTTGCTA
CAACGCTGACTTTGAGAAGCTGAAGCCAGGGTACCTGGAGCAACTCCCTGGAATGATGAGGCTTTACTCT
GAGTTCCTGGGCAAGCGGCCATGGTTCGCAGGGGACAAGATCACCTTTGTGGATTTCATTGCTTACGATG
TGCTTGAGAGGAACCAAGTGTTTGAGGCCAAGTGCCTGGATGCGTTCCCAAACCTGAAGGACTTCATAGC
GCGCTTTGAGGGCCTGAAGAAGATCTCCGACTACATGAAGACCAGTCGCTTCCTCCCAAGACCCATGTTC
ACAAAGATGGCAACTTGGGGCAGCAATTAGGACCTTGACAGGGTGGGCTGTAGGATAAAGATACCAGATC
TCCTGAGTTTGCCAGGAGCCCTGGGGTGCAGGCAGGATCCCTGCAGCCCCACAGCCAACTTTTCCTTCTT
CTTTCCTTCCATCCTGTTCCAGATCCCAAGCCTCACCGGCTCTCGTTTTTGGTCATCCAATCTCTGCCCA
Notes:The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. Forward Primer - name:unspecified, sequence:ATCCGTTTCTGGCAGCTCTC; Reverse Primer - name:unspecified, sequence:TGGGCAGAGATTGGATGACC. The reverse primer contains a 5' extension containing an unspecified RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using unspecified polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:MGI:4825266 same experiment
 EurExpress:euxassay_018910 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS