Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:10233

Acsl6 acyl-CoA synthetase long-chain family member 6 ( MGI:894291)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:10233 EMAGE:10233 EMAGE:10233 EMAGE:10233 EMAGE:10233
"Pseudo-wholemount" of euxassay_018903. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_018903_01 euxassay_018903_02 euxassay_018903_03 euxassay_018903_04
EMAGE:10233 EMAGE:10233 EMAGE:10233 EMAGE:10233 EMAGE:10233
euxassay_018903_05 euxassay_018903_06 euxassay_018903_07 euxassay_018903_08 euxassay_018903_09
EMAGE:10233 EMAGE:10233 EMAGE:10233 EMAGE:10233 EMAGE:10233
euxassay_018903_10 euxassay_018903_11 euxassay_018903_12 euxassay_018903_13 euxassay_018903_14
EMAGE:10233 EMAGE:10233 EMAGE:10233 EMAGE:10233 EMAGE:10233
euxassay_018903_15 euxassay_018903_16 euxassay_018903_17 euxassay_018903_18 euxassay_018903_19
EMAGE:10233 EMAGE:10233 EMAGE:10233 EMAGE:10233 EMAGE:10233
euxassay_018903_20 euxassay_018903_21 euxassay_018903_22 euxassay_018903_23 euxassay_018903_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:10233Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
10233_wholemount_strong.wlz
10233_wholemount_moderate.wlz
10233_wholemount_weak.wlz
10233_wholemount_possible.wlz
10233_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:10233_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
nasal cavity olfactory epithelium
strong strong
single cellstrong expression: see section 11 12 15 moderate expression: see section 09 10 16 17
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T50064
Entity Detected:Acsl6, acyl-CoA synthetase long-chain family member 6 ( MGI:894291)
Sequence:sense strand is shown

>T50064
TTCTCTCTGGCTGTTTGCCGAGATTGCTCTCTCACTTCTGGAGAAGATGCAGACCCAGGAGATCCTGAGG
ATCCTGCGGCTGCCCGAGCTATCGGACTTGGGCCAGTTTTTCCGCAGCCTCTCAGCTACCACCCTCGTGA
GTGTGGGTGCACTGGCTGCCGTCCTTGCCTACTGGCTCACTCACCGGCCAAAGGCCTTGCAACCACCATG
CAACCTCCTGAAGCAGTCGGAAGAAGTGGAGGACGGTGGTGGAGCCCGGCGATCTGTGATTGGGGGTTGC
ACTCAATTGCTTACCCATTACTATGACGATGCCCGGACCATGTACCAGGTGTTCCGCCGTGGGCTTAGCA
TCTCAGGGAATGGACCCTGTCTTGGCTTCCGGAAGCCAGAGCAACCTTACCAGTGGCTGTCCTACCAAGA
GGTGGCCAAAAGGGCTGAATTTCTGGGGTCGGGGCTTCTCCAGCACGACTGTAAAGTGGGCACAGAGCAG
TTCGTTGGTGTTTTTGCACAAAATCGGCCGGAGTGGATCATTGCCGAGCTGGCCTGCTACACGTATTCCA
TGGTGGTGGTACCGCTCTACGACACCCTGGGCCCAGGGTCTATCAGCTACATCATCAATACTGCGGACAT
CTGCACGGTAATCGTCGATAAACCCCACAAGGCAACACTTCTGCTGGAACACGTGGAGAGGAAGGAGACT
CCGGGCCTCAAGCTGGTCATCCTCATGGAGCCGTTTGAGGACGCCCTGAGAGAGAGAGGAAAGAAGTGCG
GGGTGGACATCAAGTCCATGCAGGCTATAGAGGACTGTGGCCGAGAGAATCATCATGCCCCCGTGCCCCC
ACGGCCCGATGACCTCTCCATCGTGTGTTTCACAAGTGGTACAACAGGGAACCCCAAAGGTGCGATGCTC
ACCCACGGGAACGTGGTGGCCGATTTCTCGGGCTTTCTGAAAGTGACAGAGAAAGTGATCTTTCCGAGAC
AGGACGATGTGCTCA
Notes:The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. Forward Primer - name:unspecified, sequence:TTCTCTCTGGCTGTTTGCCG; Reverse Primer - name:unspecified, sequence:TGAGCACATCGTCCTGTCTC. The reverse primer contains a 5' extension containing an unspecified RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using unspecified polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:10232 same embryo
 EMAGE:10231 same embryo
 EurExpress:euxassay_018903 same experiment
 MGI:4822946 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS