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EMAGE:10241

Gstm1 glutathione S-transferase, mu 1 ( MGI:95860)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:10241 EMAGE:10241 EMAGE:10241 EMAGE:10241 EMAGE:10241
"Pseudo-wholemount" of euxassay_018918. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_018918_01 euxassay_018918_02 euxassay_018918_03 euxassay_018918_04
EMAGE:10241 EMAGE:10241 EMAGE:10241 EMAGE:10241 EMAGE:10241
euxassay_018918_05 euxassay_018918_06 euxassay_018918_07 euxassay_018918_08 euxassay_018918_09
EMAGE:10241 EMAGE:10241 EMAGE:10241 EMAGE:10241 EMAGE:10241
euxassay_018918_10 euxassay_018918_11 euxassay_018918_12 euxassay_018918_13 euxassay_018918_14
EMAGE:10241 EMAGE:10241 EMAGE:10241 EMAGE:10241 EMAGE:10241
euxassay_018918_15 euxassay_018918_16 euxassay_018918_17 euxassay_018918_18 euxassay_018918_19
EMAGE:10241 EMAGE:10241 EMAGE:10241 EMAGE:10241 EMAGE:10241
euxassay_018918_20 euxassay_018918_21 euxassay_018918_22 euxassay_018918_23 euxassay_018918_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:10241Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
10241_wholemount_strong.wlz
10241_wholemount_moderate.wlz
10241_wholemount_weak.wlz
10241_wholemount_possible.wlz
10241_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:10241_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
thymus primordium
moderate moderate
regionalmoderate expression: see section 12 13 16 17 weak expression: see section 14 15
pancreas
weak weak
regionalweak expression: see section 10 11 12 13
hypothalamus ventricular layer
weak weak
regionalweak expression: see section 12
diencephalon lateral wall ventricular layer
weak weak
regionalweak expression: see section 10 11 12
telencephalon ventricular layer
weak weak
regionalweak expression: see section 01 02 03 04 05 06 07 08 09 11 12 17 18 19 20 21
medulla oblongata basal plate ventricular layer
weak weak
regionalweak expression: see section 11 12 14
pons ventricular layer
weak weak
regionalweak expression: see section 06 07 08 09 10 11 14 15 16 18 19
midbrain ventricular layer
moderate moderate
regionalmoderate expression: see section 08 weak expression: see section 07 09 10 11 12 13
ventral grey horn
weak weak
regionalweak expression: see section 12 13 14 15 16 17 18 19
nasal cavity olfactory epithelium
moderate moderate
regionalmoderate expression: see section 07 08 09 10 11 12 14 15 16 17 18
stomach
moderate moderate
regionalmoderate expression: see section 06 07 08 09 10
midgut
moderate moderate
regionalmoderate expression: see section 06 07 08 09 10 11 weak expression: see section 12 13 14 15 16 17 18 19
liver
moderate moderate
regionalmoderate expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 weak expression: see section 02 03
urinary system
strong strong
regionalstrong expression: see section 07 08 24
testis
moderate moderate
regionalmoderate expression: see section 07 08 09 10 20 21 22 23 24
nucleus pulposus
moderate moderate
regionalmoderate expression: see section 16
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T50465
Entity Detected:Gstm1, glutathione S-transferase, mu 1 ( MGI:95860)
Sequence:sense strand is shown

>T50465
TGCCTAATTGGGATTGGTGCAGGGTTGGGAGGGACCCGCTGTTTTGTCCTGCCCACGTTTCTCTAGTAGT
CTGTATAAAGTCACAACTCCAAACACACAGGTCAGTCCTGCTGAAGCCAGTTTGAGAAGACCACAGCACC
AGCACCATGCCTATGATACTGGGATACTGGAACGTCCGCGGACTGACACACCCGATCCGCATGCTCCTGG
AATACACAGACTCAAGCTATGATGAGAAGAGATACACCATGGGTGACGCTCCCGACTTTGACAGAAGCCA
GTGGCTGAATGAGAAGTTCAAGCTGGGCCTGGACTTTCCCAATCTGCCTTACTTGATCGATGGATCACAC
AAGATCACCCAGAGCAATGCCATCCTGCGCTACCTTGCCCGAAAGCACCACCTGGATGGAGAGACAGAGG
AGGAGAGGATCCGTGCAGACATTGTGGAGAACCAGGTCATGGACACCCGCATGCAGCTCATCATGCTCTG
TTACAACCCTGACTTTGAGAAGCAGAAGCCAGAGTTCTTGAAGACCATCCCTGAGAAAATGAAGCTCTAC
TCTGAGTTCCTGGGCAAGAGGCCATGGTTTGCAGGGGACAAGGTCACCTATGTGGATTTCCTTGCTTATG
ACATTCTTGACCAGTACCGTATGTTTGAGCCCAAGTGCCTGGACGCCTTCCCAAACCTGAGGGACTTCCT
GGCCCGCTTCGAGGGCCTCAAGAAGATCTCTGCCTACATGAAGAGTAGCCGCTACATCGCAACACCTATA
TTTTCAAAGATGGCCCACTGGAGTAACAAGTAGGCCCTTGCTACACGGGCACTCACTAGGAGGACCTGTC
CACACTGGGGATCCTGCAGGCCCTGGGTGGGGACAGCACCCTGGCCTTCTGCACTGTGGCTCCTGGTTCT
CTCTCCTTCCCGCTCCCTTCTGCAGCTTGGTCAGCCCCATCTCCTCACCCTCTTCCCAGTCAAGTCCACA
CAGCCTTCATTCTCCCCAGTTTCTTTCACATGGCCCCTTCTTCATTGGCTCCCTGACCCAACCTCACAGC
CCGTTT
Notes:The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. Forward Primer - name:unspecified, sequence:TGCCTAATTGGGATTGGTGC; Reverse Primer - name:unspecified, sequence:AAACGGGCTGTGAGGTTGGG. The reverse primer contains a 5' extension containing an unspecified RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using unspecified polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:10243 same embryo
 EMAGE:10240 same embryo
 EMAGE:10238 same embryo
 EMAGE:10242 same embryo
 EMAGE:10239 same embryo
 EurExpress:euxassay_018918 same experiment
 MGI:4825260 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS