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EMAGE:10260

Cox10 COX10 homolog, cytochrome c oxidase assembly protein, heme A: farnesyltransferase (yeast) ( MGI:1917633)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:10260 EMAGE:10260 EMAGE:10260 EMAGE:10260 EMAGE:10260
"Pseudo-wholemount" of euxassay_018930. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_018930_01 euxassay_018930_02 euxassay_018930_03 euxassay_018930_04
EMAGE:10260 EMAGE:10260 EMAGE:10260 EMAGE:10260 EMAGE:10260
euxassay_018930_05 euxassay_018930_06 euxassay_018930_07 euxassay_018930_08 euxassay_018930_09
EMAGE:10260 EMAGE:10260 EMAGE:10260 EMAGE:10260 EMAGE:10260
euxassay_018930_10 euxassay_018930_11 euxassay_018930_12 euxassay_018930_13 euxassay_018930_14
EMAGE:10260 EMAGE:10260 EMAGE:10260 EMAGE:10260 EMAGE:10260
euxassay_018930_15 euxassay_018930_16 euxassay_018930_17 euxassay_018930_18 euxassay_018930_19
EMAGE:10260 EMAGE:10260 EMAGE:10260 EMAGE:10260 EMAGE:10260
euxassay_018930_20 euxassay_018930_21 euxassay_018930_22 euxassay_018930_23 euxassay_018930_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:10260Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
10260_wholemount_strong.wlz
10260_wholemount_moderate.wlz
10260_wholemount_weak.wlz
10260_wholemount_possible.wlz
10260_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:10260_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
thymus primordium
weak weak
regionalweak expression: see section 11 12 13 14 15
submandibular gland primordium
weak weak
regionalweak expression: see section 06 07 08 09 17 18 19
esophagus
weak weak
regionalweak expression: see section 12 13
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T50255
Entity Detected:Cox10, COX10 homolog, cytochrome c oxidase assembly protein, heme A: farnesyltransferase (yeast) ( MGI:1917633)
Sequence:sense strand is shown

>T50255
ACCATTCCCTCGACACAGCCAAAGAGAAAAAGGAAGAAAGGCAGTGGAAAGAGATGAAACTTCACACTGA
TGATCTGCCTGGCATTTTGGCCAGGCTGTCCAAGATCAAACTCACAGCTCTTGTGGTAAGCACCACTTCA
GCAGGCTTTGCACTGGCTCCAGGCCCTTTCGACTGGTCCTGCTTCCTGCTTACATCCCTTGGAACCGGCC
TCGCATCCTGTGCTGCCAACTCCATCAATCAGTTTTTTGAGGTGCCGTTCGACTCAAACATGAATAGAAC
AAAGAACAGGCCTCTGGTTCGAGGGCAGATAAGCCCATTGCTAGCCGTGTCTTTTGCCACCTGCTGTGCT
GTGCCAGGAGTGGCCCTTCTGACCTGGGGAGTGAATCCACTCACAGGGGCCTTGGGGGTCTTCAACATTT
TCCTGTATACCTGTTGTTACACACCACTGAAGAGGGTCAGCATCACCAATACCTGGGTTGGAGCCGTGGT
TGGAGCCATCCCTCCTGTCATGGGCTGGACGGCCGCCACTGGAAGCCTTGATGCTGGAGCGCTTCTTCTG
GGAGGGATCCTGTACTCCTGGCAATTCCCCCACTTCAATGCCCTGAGCTGGGGCCTCCGTGAAGACTACT
CCCGAGGAGGCTACTGTATGATGTCCGTCACCCATCCAGCCCTCTGCCGGCGGGTGGCACTGCGCCACTG
CCTGGCCCTGATTGCTCTGTCGACTGCTGCCCCGGTCCTGGACATCACCACCTGGGTCTTCCCTGTCATC
TCCCTCCCCATCAACCTGTACATTTCCT
Notes:The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. Forward Primer - name:unspecified, sequence:ACCATTCCCTCGACACAGCC; Reverse Primer - name:unspecified, sequence:AGGAAATGTACAGGTTGATG. The reverse primer contains a 5' extension containing an unspecified RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using unspecified polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:MGI:4824015 same experiment
 EurExpress:euxassay_018930 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS