EMAGE mouse gene expression spatial database

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EMAGE:10262

Paics phosphoribosylaminoimidazole carboxylase, phosphoribosylaminoribosylaminoimidazole, succinocarboxamide synthetase ( MGI:1914304)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_018936. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_018936_01	euxassay_018936_02	euxassay_018936_03	euxassay_018936_04

euxassay_018936_05	euxassay_018936_06	euxassay_018936_07	euxassay_018936_08	euxassay_018936_09

euxassay_018936_10	euxassay_018936_11	euxassay_018936_12	euxassay_018936_13	euxassay_018936_14

euxassay_018936_15	euxassay_018936_16	euxassay_018936_17	euxassay_018936_18	euxassay_018936_19

euxassay_018936_20

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10262_wholemount_strong.wlz

10262_wholemount_moderate.wlz

10262_wholemount_weak.wlz

10262_wholemount_possible.wlz

10262_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10262_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

submandibular gland primordium

weak

regional

weak expression: see section 06 07 08 09 18 19 20

vibrissa

weak

regional

weak expression: see section 03 04 06 18 19 20

hypothalamus ventricular layer

moderate

homogeneous

moderate expression: see section 11 12 13 14

diencephalon lateral wall ventricular layer

moderate

homogeneous

moderate expression: see section 11 12 13 14

olfactory cortex ventricular layer

strong

homogeneous

strong expression: see section 15 16 moderate expression: see section 09 10

telencephalon ventricular layer

strong

homogeneous

strong expression: see section 15 16 20 moderate expression: see section 01 02 03 04 08 09 10 11 13 14 18 19 weak expression: see section 05 06 07 17

rest of cerebellum marginal layer

strong

regional

strong expression: see section 18 19 moderate expression: see section 04 05 06 07 08 09 weak expression: see section 10 11 12 14 16 17

rest of cerebellum ventricular layer

strong

regional

strong expression: see section 18 19 moderate expression: see section 04 05 06 07 08 09 weak expression: see section 12 17

midbrain ventricular layer

moderate

regional

moderate expression: see section 09 10 11 12 13 14 15 16 17 weak expression: see section 18

nucleus pulposus

strong

regional

strong expression: see section 14 moderate expression: see section 15

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T50849
Entity Detected:	Paics, phosphoribosylaminoimidazole carboxylase, phosphoribosylaminoribosylaminoimidazole, succinocarboxamide synthetase ( MGI:1914304)
Sequence:	sense strand is shown >T50849 AGGACTTGGTCCGCTCCAACCCAGAAACCTCTCCGGCCAGCGGGGGCCTTGCAGCATGCGCGCCACTTTT TGGCTGCCTGCGGGGAGGGGCCGCCCGGAAGCGGGGTCGCGTCAGCGCGCCTGCGCGGCGGGAAGCCTGG GGACACGACTCCAGCCCCAAGCACAGCCAGCCCGGTACTTCGGGTTCGCCGGTGGGCTGTGGGGCTAGGG CGTGTCCTTGATATTGCACCTCCCTTTCCCGCCCTCTGATCCTGTGGTCGCCCTTGTTTTCCAGAACACT GCCCTTTTTTCCCGCGCGGTCTGCTCCTCTAGTTCACCTCGGATAATGGCGACAGCCGTAGTAGTGAACA TCGGGAAGAAGCTCTACGAGGGTAAAACAAAAGAAGTCTATGAGTTGTTAGACACCCCAGGCAGAGTCCT CCTGCAGTCCAAGGACCAGATCACAGCAGGGAATGCAGCTAGGAAGAACCACCTGGAAGGCAAAGCCGCC ATCTCCAACAAGATCACCAGCTGCATATTTCAGTTGTTACAGGAAGCTGGTATAAAAACTGCTTTCACCA AGAAATGCGGGGAGACTGCTTTCATTGCACCCCAGTGTGAAATGATCCCCATTGAGTGGGTGTGCCGAAG AATAGCAACTGGGTCTTTCCTCAAAAGGAACCCTGGGGTACAGGAGGGGTACAAGTTCTATCCACCCAAA GTGGAGATGTTCTTCAAGGACGATGCCAATAATGATCCACAGTGGTCTGAGGAGCAACTCATTGCTGCAA AATTCTGTTTTGCTGGCCTTGTTATAGGGCAGACT
Notes:	The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. Forward Primer - name:unspecified, sequence:AGGACTTGGTCCGCTCCAAC; Reverse Primer - name:unspecified, sequence:AGTCTGCCCTATAACAAGGC. The reverse primer contains a 5' extension containing an unspecified RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using unspecified polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10263 same embryo
	EMAGE:10264 same embryo
	EurExpress:euxassay_018936 same experiment
	MGI:4827027 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source