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EMAGE:10263

Pgk1 phosphoglycerate kinase 1 ( MGI:97555)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:10263 EMAGE:10263 EMAGE:10263 EMAGE:10263 EMAGE:10263
"Pseudo-wholemount" of euxassay_018885. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_018885_01 euxassay_018885_02 euxassay_018885_03 euxassay_018885_04
EMAGE:10263 EMAGE:10263 EMAGE:10263 EMAGE:10263 EMAGE:10263
euxassay_018885_05 euxassay_018885_06 euxassay_018885_07 euxassay_018885_08 euxassay_018885_09
EMAGE:10263 EMAGE:10263 EMAGE:10263 EMAGE:10263 EMAGE:10263
euxassay_018885_10 euxassay_018885_11 euxassay_018885_12 euxassay_018885_13 euxassay_018885_14
EMAGE:10263 EMAGE:10263 EMAGE:10263 EMAGE:10263 EMAGE:10263
euxassay_018885_15 euxassay_018885_16 euxassay_018885_17 euxassay_018885_18 euxassay_018885_19
EMAGE:10263
euxassay_018885_20

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:10263Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
10263_wholemount_strong.wlz
10263_wholemount_moderate.wlz
10263_wholemount_weak.wlz
10263_wholemount_possible.wlz
10263_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:10263_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T50805
Entity Detected:Pgk1, phosphoglycerate kinase 1 ( MGI:97555)
Sequence:sense strand is shown

>T50805
CTCCGGAGGCCCGGCATTCTGCACGCTTCAAAAGCGCACGTCTGCCGCGCTGTTCTCCTCTTCCTCATCT
CCGGGCCTTTCGACCTCACGGTGTTGCCAAAATGTCGCTTTCCAACAAGCTGACTTTGGACAAGCTGGAC
GTGAAGGGGAAGCGGGTCGTGATGAGGGTGGACTTCAACGTTCCTATGAAGAACAACCAGATAACAAACA
ACCAAAGGATCAAGGCTGCTGTTCCAAGCATCAAATTCTGCTTGGACAATGGAGCCAAGTCCGTTGTCCT
TATGAGCCACCTGGGCCGGCCTGATGGTGTTCCCATGCCTGACAAGTACTCCTTAGAGCCAGTTGCTGCT
GAACTCAAATCTCTGCTGGGCAAGGATGTTCTGTTCTTGAAGGATTGTGTGGGCCCAGAAGTCGAGAATG
CCTGTGCCAACCCAGCGGCTGGGACTGTCATCCTGCTGGAAAACCTCCGCTTTCATGTAGAGGAAGAAGG
GAAGGGAAAAGATGCTTCTGGAAACAAGGTTAAAGCTGAGCCGGCCAAAATTGATGCTTTCCGAGCCTCA
CTGTCCAAACTAGGAGATGTCTATGTCAATGATGCTTTTGGGACTGCACACCGAGCCCATAGCTCCATGG
TGGGTGTGAATCTGCCACAGAAGGCTGGTGGATTTTTGATGAAGAAGGAGCTGAACTACTTTGCCAAGGC
TTTGGAGAGTCCAGAGCGACCCTTCCTGGCTATCTTGGGAGGCGCTAAAGTTGCAGACAAGATCCAGCTG
ATCAATAATATGCTAGACAAAGTCAATGAGATGATCATTGGTGGTGGAATGGCCTTTACCTTCCTTAAGG
TGCTCAACAACATGGAGATTGGCACATCTCTGTATGATGAAGAAGGAGCCAAGATTGTCAA
Notes:The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. Forward Primer - name:unspecified, sequence:unspecified; Reverse Primer - name:unspecified, sequence:unspecified. The reverse primer contains a 5' extension containing an unspecified RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using unspecified polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:10264 same embryo
 EMAGE:10262 same embryo
 EurExpress:euxassay_018885 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS