EMAGE mouse gene expression spatial database

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EMAGE:10267

Atp13a2 ATPase type 13A2 ( MGI:1922022)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_018927. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_018927_01	euxassay_018927_02	euxassay_018927_03	euxassay_018927_04

euxassay_018927_05	euxassay_018927_06	euxassay_018927_07	euxassay_018927_08	euxassay_018927_09

euxassay_018927_10	euxassay_018927_11	euxassay_018927_12	euxassay_018927_13	euxassay_018927_14

euxassay_018927_15	euxassay_018927_16	euxassay_018927_17	euxassay_018927_18	euxassay_018927_19

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10267_wholemount_strong.wlz

10267_wholemount_moderate.wlz

10267_wholemount_weak.wlz

10267_wholemount_possible.wlz

10267_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10267_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

weak

regional

weak expression: see section 09 10

submandibular gland primordium

weak

regional

weak expression: see section 08 12 13 14 15

vibrissa

moderate

regional

moderate expression: see section 07 08 weak expression: see section 18 19

facial vii ganglion

strong

regional

strong expression: see section 04 05 06 15 16 17

glossopharyngeal ix ganglion

strong

regional

strong expression: see section 06 07 13 14 moderate expression: see section 12

trigeminal v ganglion

strong

regional

strong expression: see section 03 04 05 06 07 08 13 14 15 16 17 18

vagus x ganglion

strong

regional

strong expression: see section 08

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 14

ventral grey horn

weak

regional

weak expression: see section 09 10 11 12 13

dorsal root ganglion

strong

regional

strong expression: see section 06 07 09 10 11 12 13 14 moderate expression: see section 08

neural retina

moderate

regional

moderate expression: see section 04 05 weak expression: see section 02 03

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T50136
Entity Detected:	Atp13a2, ATPase type 13A2 ( MGI:1922022)
Sequence:	sense strand is shown >T50136 TCGGCTATCACGCCGCGGTCTGGATGCTGGCCGGGATCCCTTGGCTGCTATTCCGCTGGAAGCCCTTGTG GGGCGTGCGCCTGCGCCTGAAGCCATGCAGCCTGGCCCACGCCGAAACACTCGTTATAGAAATAAAAGAC AAAGAGGGTAGCTCCAGACAGCTCTTCACGGTTCAGGTGCAGACTGAGGCTCTTGTCCAGGGCAGCCTGG AGCTGCCCCCACAGGCCCAGGCAGAGGATGGCCGGAGCCAGGCAGCCGTGGGAGTGACACCAGAGGGCAC ATGGCAGGACACCTCCGAACTCCACAGGCAGGAAGAGGCAAAGCAGGTACTGCGCTATTATGTCCTCCAA GGTCAACGCTACGTCTGGATGGAAACCCAGCAAGCCTTCTGCCAAGTCAGCCTGCTGGACCACAGTCGTA CCTGTGATGACGTCCACTGCTCCAGCTCCGGCCTCAGCCTCCAAGACCAAGCTACGAGGAAGACCATTTA CGGCCCCAATGTGATCAGTATTCCTGTCAAGTCCTACCTGCAGCTGCTGGCGGATGAGGCCCTGAATCCC TACTATGGGTTCCAAGCCTTCAGCATCGCACTGTGGCTGGCCGACCACTACTACTGGTATGCTCTCTGCA TCTTCCTCATCTCAGCCATCTCCATCTGCCTGGCTCTGTACAAGACCAGAAAGCAAAGCTTGACCCTGAG GGACATGGTCAAGCTGTCCGTGCGGGTGCAGGTGTGCCGACCCGGAGGAGAGGAAGAGTGGGTGGACTCC AGTGAGCTGGTGCCCGGAGACTGCCTGGTGCTGCCCCAGGAGGGCGGGGTGATGCCATGCGACGCGGCTC TGGTGGCCGGGGAGTGTGTGGTCAACGAGAGCT
Notes:	The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. Forward Primer - name:unspecified, sequence:TCGGCTATCACGCCGCGGTC; Reverse Primer - name:unspecified, sequence:AGCTCTCGTTGACCACACAC. The reverse primer contains a 5' extension containing an unspecified RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using unspecified polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10265 same embryo
	EMAGE:10266 same embryo
	EurExpress:euxassay_018927 same experiment
	MGI:4823303 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source