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EMAGE:1033

Lmo2 LIM domain only 2 ( MGI:102811)
TS11 (8.0 dpc)
in situ hybridisation

Data Images
EMAGE:1033 EMAGE:1033
Fig 1D (top) Manaia et al, 2000 [PMID:10631184] .Copyright: This image is from Development and is displayed with the permission of the Company of Biologists Ltd who owns the copyright. Fig 1D (bottom) Manaia et al, 2000 [PMID:10631184] .Copyright: This image is from Development and is displayed with the permission of the Company of Biologists Ltd who owns the copyright.

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Find spatially similar expression patterns: Find spatially similar patterns
Notes:
Image annotations: YS - yolk sac, Al - allantois. Both of these samples (C and D) are TS11 and the results from both have been annotated using text in this EMAGE entry whereas only the data from Image D (both wholemount and section) has been spatially mapped. Sample C is younger (neural plate stage) than sample D (early headfold stage). Image annotations: Arrowheads in: D= yolk sac blood islands (black); cephalic mesoderm (white); C= Lmo2-positive ring. Ab= allantoic bud, Al= allantois, YS= yolk sac.
Expression Pattern Description
Spatial Annotation:
EMAGE:1033EMAGE:1033EMAGE:1033Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
3D mapping3D context moviewholemount mapping

View mapped 3D expression image EMAGE genex expression entry
Download individual expression domains:
1033_wholemount_strong_3D_2.wlz
1033_wholemount_moderate_3D_1.wlz
1033_wholemount_weak_3D_1.wlz
1033_wholemount_possible_3D_1.wlz
1033_wholemount_notDetected_3D_2.wlz
1033_voxel_strong_3D_1.wlz
1033_voxel_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:1033_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Find spatially similar expression patterns: EMAGE spatially similar patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
head mesenchyme
detected detected
allantois
detected detected
blood island
detected detected
Annotation Validation: Submitter + EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:1351844
Entity Detected:Lmo2, LIM domain only 2 ( MGI:102811)
Notes:The probe used in this study by Manaia et al, 2000 [PMID:10631184] is described as a "500 bp cDNA fragment subcloned at the Bluescript EcoRV site". The exact sequence of this probe is not reported.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:BALB/c
Age:8.0 dpc
Theiler Stage:TS11
Mutations:none (wild-type)
Preparation:sectioned wholemount
Procedures
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Manaia et al, 2000 [PMID:10631184] Indexed by GXD, Spatially mapped by EMAGE
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:10631184] Manaia A, Lemarchandel V, Klaine M, Max-Audit I, Romeo P, Dieterlen-Lievre F, Godin I 2000 Lmo2 and GATA-3 associated expression in intraembryonic hemogenic sites. Development (127):643-53
Links:MGI:1352410 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI