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EMAGE:1034

Lmo2 LIM domain only 2 ( MGI:102811)
TS14 (9.5 dpc)
in situ hybridisation

Data Images
EMAGE:1034
Fig 1H Manaia et al, 2000 [PMID:10631184] . Copyright: This image is from Development and is displayed with the permission of the Company of Biologists Ltd who owns the copyright.

Expression pattern clarity: two stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
The authors state this embryo has 20-25 somites. Image annotations: Arrowheads = cranial capillaries (black), branchial arch (white); Arrows = truncal aorta (black), intersomitic vessels (white); H= heart; YS=yolk sac; Pvm= posterior ventral mesoderm.
Expression Pattern Description
Spatial Annotation:
EMAGE:1034Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
1034_wholemount_strong_3D_1.wlz
1034_wholemount_moderate_3D_1.wlz
1034_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:1034_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
trunk mesenchyme
detected detected
regionalExpression is in the posterior ventral mesoderm
branchial arch
detected detected
cardiovascular system
detected detected
regionalExpression is in cranial capillaries and intersomitic vessels
dorsal aorta
detected detected
regionalExpression is in the truncal aorta
extraembryonic component
weak weak
Expression is downregulated in circulating hematopoetic cells
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:1351844
Entity Detected:Lmo2, LIM domain only 2 ( MGI:102811)
Notes:The probe used in this study by Manaia et al, 2000 [PMID:10631184] is described as a "500 bp cDNA fragment subcloned at the Bluescript EcoRV site". The exact sequence of this probe is not reported.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:BALB/c
Age:9.5 dpc
Theiler Stage:TS14
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Manaia et al, 2000 [PMID:10631184] Indexed by GXD, Spatially mapped by EMAGE
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:10631184] Manaia A, Lemarchandel V, Klaine M, Max-Audit I, Romeo P, Dieterlen-Lievre F, Godin I 2000 Lmo2 and GATA-3 associated expression in intraembryonic hemogenic sites. Development (127):643-53
Links:MGI:1352410 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI