EMAGE mouse gene expression spatial database

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EMAGE:10354

Drosha drosha, ribonuclease type III ( MGI:1261425)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_018939. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_018939_01	euxassay_018939_02	euxassay_018939_03	euxassay_018939_04

euxassay_018939_05	euxassay_018939_06	euxassay_018939_07	euxassay_018939_08	euxassay_018939_09

euxassay_018939_10	euxassay_018939_11	euxassay_018939_12	euxassay_018939_13	euxassay_018939_14

euxassay_018939_15	euxassay_018939_16	euxassay_018939_17	euxassay_018939_18	euxassay_018939_19

euxassay_018939_20	euxassay_018939_21	euxassay_018939_22	euxassay_018939_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10354_wholemount_strong.wlz

10354_wholemount_moderate.wlz

10354_wholemount_weak.wlz

10354_wholemount_possible.wlz

10354_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10354_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

submandibular gland primordium

weak

regional

weak expression: see section 06 07 08 15 16 17

vibrissa

weak

regional

weak expression: see section 05 06 07 21 22

lower jaw incisor

weak

regional

weak expression: see section 10 11 12 14 15

lower jaw molar

weak

regional

weak expression: see section 06 19

upper jaw incisor

weak

regional

weak expression: see section 11 12 15 16

upper jaw molar

weak

regional

weak expression: see section 06 19

metanephros

weak

regional

weak expression: see section 05 06 07 08 14 15 16 17 18

lung

weak

regional

weak expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T50934
Entity Detected:	Drosha, drosha, ribonuclease type III ( MGI:1261425)
Sequence:	sense strand is shown >T50934 GAATAGGCAAGAGAAGGCCAAAGCTGCCCGGCCTCCGTGGGAGCCTCCGAAGACAAAGCTGGATGAAGAT TTAGAGAGTTCCAGTGAGTCTGAATGTGAGACTGACGACGACAGCACCTGTTCGAGCAGCTCGGACTCGG AGGTGTTCGATGTCATTGCAGAGATTAAACGCAAAAAGGCTCACCCTGACCGGCTTCATGATGAACTCTG GTACAACGACCCAGGCCAGATGAACGATGGACCGCTTTGCAAATGCAGTGCAAAAGCCAGACGCACAGGA ATCCGCCACAGCATTTATCCCGGAGAAGAGGCAATCAAGCCCTGCCGTCCGATGACCAACAACGCTGGCC GGCTTTTCCACTATCGGATCACCGTCTCCCCGCCTACCAACTTCTTAACTGACAGGCCAACAGTCATAGA ATATGATGACCACGAGTATATTTTTGAAGGATTTTCTATGTTTGCACATGCTCCTCTGACCAATATTCCA CTGTGTAAAGTGATTCGATTCAACATAGACTACACGATTCATTTTATCGAAGAGATGATGCCTGAGAATT TTTGTGTGAAAGGACTTGAACTGTTTTCATTGTTCCTATTCAGAGATATTTTGGAATTATATGACTGGAA TCTTAAAGGTCCTTTGTTTGAAGACAGCCCTCCCTGCTGTCCGAGATTTCATTTCATGCCACGTTTTGTA AGATTTCTTCCAGATGGAGGCAAAGAAGTGTTATCCATGCACCAGATCCTTCTCTACCTGCTGCGCTGCA GCAAGGCTCTGGTGCCCGAGGAGGAGATTGCCAACATGCTCCAGTGGGAGGAGCTCGAGTGGCAGAAATA CGCAGAGGAGTGCAAAGGCATGATCGTTACCAACCCTGGGACGAAACCGAGCTCTGTCCGCATTGATCAG CTGGATCGTGAACAGTTCAACCCCGAAGTGAT
Notes:	The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. Forward Primer - name:unspecified, sequence:GAATAGGCAAGAGAAGG; Reverse Primer - name:unspecified, sequence:ATCACTTCGGGGTTGAACTG. The reverse primer contains a 5' extension containing an unspecified RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using unspecified polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10353 same embryo
	EMAGE:10355 same embryo
	EurExpress:euxassay_018939 same experiment
	MGI:4827756 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source