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EMAGE:10356

Des desmin ( MGI:94885)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:10356 EMAGE:10356 EMAGE:10356 EMAGE:10356 EMAGE:10356
"Pseudo-wholemount" of euxassay_003335. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_003335_01 euxassay_003335_02 euxassay_003335_03 euxassay_003335_04
EMAGE:10356 EMAGE:10356 EMAGE:10356 EMAGE:10356 EMAGE:10356
euxassay_003335_05 euxassay_003335_06 euxassay_003335_07 euxassay_003335_08 euxassay_003335_09
EMAGE:10356 EMAGE:10356 EMAGE:10356 EMAGE:10356 EMAGE:10356
euxassay_003335_10 euxassay_003335_11 euxassay_003335_12 euxassay_003335_13 euxassay_003335_14
EMAGE:10356 EMAGE:10356 EMAGE:10356 EMAGE:10356 EMAGE:10356
euxassay_003335_15 euxassay_003335_16 euxassay_003335_17 euxassay_003335_18 euxassay_003335_19
EMAGE:10356 EMAGE:10356 EMAGE:10356 EMAGE:10356 EMAGE:10356
euxassay_003335_20 euxassay_003335_21 euxassay_003335_22 euxassay_003335_23 euxassay_003335_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:10356Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
10356_wholemount_strong.wlz
10356_wholemount_moderate.wlz
10356_wholemount_weak.wlz
10356_wholemount_possible.wlz
10356_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:10356_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
diaphragm
strong strong
homogeneousstrong expression: see section 01 02 03 04 05 06 07 08 09 10 19 20 21 22 23 24
hand mesenchyme
strong strong
regionalstrong expression: see section 01 02 03 04 22 23 24
foot mesenchyme
strong strong
regionalstrong expression: see section 01 02 03 04 05 21 22 23 24
head mesenchyme
strong strong
regionalstrong expression: see section 01 02 03 04 05 06 07 08 09 10 11 16 17 18 19 20 21 22 23 24
trunk mesenchyme
strong strong
regionalstrong expression: see section 03 04 05 06 07 08 09 10 11 18 19 20 21 22 23
vertebral axis musculature
strong strong
regionalstrong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24
tongue
strong strong
regionalstrong expression: see section 10 11 12 13 14 15
tongue muscle
strong strong
regionalstrong expression: see section 10 11 12 13 14 15
tail mesenchyme
strong strong
regionalstrong expression: see section 11 12 13 14 15 16 17
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T6382
Entity Detected:Des, desmin ( MGI:94885)
Sequence:sense strand is shown

>T6382
CCTGAAGCCAGCCTCTTCCACTCTCGGATATCACACCCAGCCACTTTTCTCCACTCACAGGCTCTGACCC
CCCCTCACCGATCACCCCTTTGTGGTCTTCATGCTGCCCAACCCCAGGGAACCCCTCAGCCACCTCTGCA
GACCCTCCCATGAGCCCTGGCTATTGGCAGGTGTCAAAGCTGGCTCTTAAGAGAGAACCCAGCTCAAGTC
ATCGCCCTTCCCCTTCCACCTTTGTGACCCCTGGCTTAGGAGAGGGTACCAGAGAGGGTGTTGGGATCTG
CAGGGTCAGGACCGAGTTTGTGGACATCCCCAGCCTGGGTCAGAGACAGAATGAAGCCTCAGCGAGCTGA
GATGGAGAGTGGGGGGCCTGAAAACTGCCCTCATGGCCCCTCTCTTTCCCATCGCAGCCCAGGATGGCCT
TGGAAAGCGGGGGCTGTAAGAGGGAAGCGGAAGGTGCTGGATGTGGGAGCAGGAGCTACAGAAGGAGAGA
GGATGGGTGAGGAGCTGGAGAGGAAGGAAGAGAGAGGCAGAGAGTGGGCTCAGGT
Notes:The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. Forward Primer - name:unspecified, sequence:AACAACATGAAGTGCTGTAAG; Reverse Primer - name:unspecified, sequence:TTGTGCTTGTCTCTTTATTATTG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:10361 same embryo
 EMAGE:10358 same embryo
 EMAGE:10357 same embryo
 EMAGE:10360 same embryo
 EMAGE:10359 same embryo
 EurExpress:euxassay_003335 same experiment
 MGI:4824284 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS