EMAGE mouse gene expression spatial database

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EMAGE:10366

Chrna1 cholinergic receptor, nicotinic, alpha polypeptide 1 (muscle) ( MGI:87885)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_000682. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_000682_01	euxassay_000682_02	euxassay_000682_03	euxassay_000682_04

euxassay_000682_05	euxassay_000682_06	euxassay_000682_07	euxassay_000682_08	euxassay_000682_09

euxassay_000682_10	euxassay_000682_11	euxassay_000682_12	euxassay_000682_13	euxassay_000682_14

euxassay_000682_15	euxassay_000682_16	euxassay_000682_17	euxassay_000682_18	euxassay_000682_19

euxassay_000682_20	euxassay_000682_21	euxassay_000682_22	euxassay_000682_23	euxassay_000682_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10366_wholemount_strong.wlz

10366_wholemount_moderate.wlz

10366_wholemount_weak.wlz

10366_wholemount_possible.wlz

10366_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10366_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

diaphragm

moderate

regional

moderate expression: see section 01 02 03 04 05 06 21 22 23 weak expression: see section 07 08 09 10 11 20 24

limb

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 09 10 11

vertebral axis musculature

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

tongue

moderate

regional

moderate expression: see section 12 13 14 15 weak expression: see section 16 17 18 19

pectoral girdle and thoracic body wall skeleton

moderate

regional

moderate expression: see section 06 07 08 09 10 12 13 14 17 18 19 20 21 22 23

tail

moderate

regional

moderate expression: see section 15 16 17 18 19 20

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T3763
Entity Detected:	Chrna1, cholinergic receptor, nicotinic, alpha polypeptide 1 (muscle) ( MGI:87885)
Sequence:	sense strand is shown >T3763 TGGCCTCGAGCAGATTCGGACGAGGGGATTTTTACAGAAGACATAGATATATCTGACATCTCTGGGAAGC CGGGTCCTCCACCTATGGGCTTTCACTCTCCGCTGATCAAGCACCCTGAGGTGAAAAGCGCCATCGAGGG CGTGAAGTACATTGCAGAGACCATGAAGTCAGACCAGGAGTCCAATAACGCCGCTGAGGAATGGAAGTAT GTTGCCATGGTGATGGATCACATCCTCCTCGGAGTCTTTATGCTGGTGTGTCTCATCGGGACGCTGGCTG TGTTTGCAGGTCGGCTCATTGAGTTACATCAACAAGGATGAGCAGAGGCTGAGCTAAGCCTACCTCTGTC CCAGCCATAGCCATCGCTAGGAAAGATGGAAGAGAGGAAGGTCTGTCTCCTTGAATCCTTTCACACTTAC CAAACATGCAGTGTTCTACATGTCCTACATGTTAATGAGAGTGATCTCTGCTCACACGGCTGTATTCTTG AAGTGTCTCCCCTTTGCTTCTGCTTTTAACACTATGGGCCTCCTTAAAGGGCGAACCCTTTG
Notes:	The probe template was PCR amplified from IMAGE:386668 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:386668 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10365 same embryo
	EMAGE:10364 same embryo
	EurExpress:euxassay_000682 same experiment
	MGI:4823854 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source