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EMAGE:1037

Kdr kinase insert domain protein receptor ( MGI:96683)
TS12 (8.5 dpc)
in situ hybridisation

Data Images
EMAGE:1037 EMAGE:1037
Figs 4A&B Yamaguchi et al., 1993 [PMID:8223275] . Copyright: This image is from Development and is displayed with the permission of the Company of Biologists Ltd who owns the copyright. Figs 4E&F Yamaguchi et al., 1993 [PMID:8223275] . Copyright: This image is from Development and is displayed with the permission of the Company of Biologists Ltd who owns the copyright.

Expression pattern clarity: two stars
Find spatially similar expression patterns: Find spatially similar patterns
Notes:
Image annotations: ec, endocardium; hm, head mesenchyme; np, neural plate; f, foregut pocket; e, edothelial cell; b, blood island. A&E & B&F are bright- and dark-field images of the same sections. A/B is defined by the authors as a transverse section at the level of the heart, E/F is a section through yolk sac blood islands.
Expression Pattern Description
Spatial Annotation:
EMAGE:1037EMAGE:1037Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
3D mapping3D context movie

View mapped 3D expression image EMAGE genex expression entry
Download individual expression domains:
1037_voxel_strong_3D_1.wlz
1037_voxel_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:1037_all_domains.zip
Find spatially similar expression patterns: EMAGE spatially similar patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
head mesenchyme
strong strong
spottedFig 4AB
heart
detected detected
flk-1 transcripts were detectable in the endocardium (Fig 4AB).
vitelline vein embryonic part
detected detected
Fig 4 AB
blood island
detected detected
regionalExpression was seen in yolk sac mesoderm in patches of cells lining the perimeter of the blood islands. Morphology of the cells indicate that expression is mainly confined to endothelial cell precursors (Fig 4EF)
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:14717
Entity Detected:Kdr, kinase insert domain protein receptor ( MGI:96683)
Sequence:sense strand is shown

>MGI:14717
TTGGCTCACAGGCAACATCGGTCCACATGGGCGAATCACTCACACCAGTTTGCAAGAACTTGGATGCTCT
TTGGAAACTGAATGGCACCATGTTTTCTAACAGCACAAATGACATCTTGATTGTGGCATTTCAGAATGCC
TCTCTGCAGGACCAAGGCGACTATGTTTGCTCTGCTCAAGATAAGAAGACCAAGAAAAGACATTGCCTGG
TCAAACAGCTCATCATCCTAGAGCGCATGGCACCCATGATCACCGGAAATCTGGAGAATCAGACAACAAC
CATTGGCGAGACCATTGAAGTGACTTGCCCAGCATCTGGAAATCCTACCCCACACATTACATGGTTCAAA
GACAACGAGACCCTGGTAGAAGATTCAGGCATTGTACTGAGAGATGGGAACCGGAACCTGACTATCCGCA
GGGTGAGGAAGGAGGATGGAGGCCTCTACACCTGCCAGGCCTGCAATGTCCTTGGCTGTGCAAGAGCGGA
GACGCTCTTCATAATAGAAGGTGCCCAGGAAAAGACCAACTTGGAAGTCATTATCCTCGTCGGCACTGCA
GTGATTGCCATGTTCTTCTGGCTCCTTCTTGTCATTCTCGTACGGACCGTTAAGCGGGCCAATGAAGGGG
AACTGAAGACAGGCTACTTGTCTATTGTCATGGATCCAGATGAATTGCCCTTGGATGAGCGCTGTGAACG
CTTGCCTTATGATGCCAGCAAGTGG
nt 1958 - nt 2682 of X59397.1
Notes:The Kdr (flk-1) probe used in this study by Yamaguchi et al., 1993 [PMID:8223275] is described as "a partial flk-1 cDNA", "corresponding to nt 1958 to 2682 according to the nomenclature of Matthews et al, 1991 [PMID:1717995] . GenBank sequence X59397 is the same as appears in Matthews et al (in Fig 1).
Chemistry:RNA
Strand:antisense
Label:S35
Specimen
Organism:mouse
Strain:CD-1
Age:8.5 dpc
Theiler Stage:TS12
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:4% paraformaldehyde
Embedding:paraffin
Staining procedure:autoradiography
General Information
Authors:Yamaguchi et al., 1993 [PMID:8223275] Indexed by GXD, Spatially mapped by EMAGE
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:8223275] Yamaguchi TP, Dumont DJ, Conlon RA, Breitman ML, Rossant J 1993 flk-1, an flt-related receptor tyrosine kinase is an early marker for endothelial cell precursors. Development (118):489-98
 [ doi:10.1073/pnas.88.20.9026] [ PMID:1717995] Matthews W, Jordan CT, Gavin M, Jenkins NA, Copeland NG, Lemischka IR 1991 A receptor tyrosine kinase cDNA isolated from a population of enriched primitive hematopoietic cells and exhibiting close genetic linkage to c-kit. Proc Natl Acad Sci U S A (88):9026-30
Links:MGI:1277537 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI