EMAGE mouse gene expression spatial database

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EMAGE:10391

Emx1 empty spiracles homolog 1 (Drosophila) ( MGI:95387)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_019478. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_019478_01	euxassay_019478_02	euxassay_019478_03	euxassay_019478_04

euxassay_019478_05	euxassay_019478_06	euxassay_019478_07	euxassay_019478_08	euxassay_019478_09

euxassay_019478_10	euxassay_019478_11	euxassay_019478_12	euxassay_019478_13	euxassay_019478_14

euxassay_019478_15	euxassay_019478_16	euxassay_019478_17	euxassay_019478_18	euxassay_019478_19

euxassay_019478_20	euxassay_019478_21	euxassay_019478_22	euxassay_019478_23	euxassay_019478_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10391_wholemount_strong.wlz

10391_wholemount_moderate.wlz

10391_wholemount_weak.wlz

10391_wholemount_possible.wlz

10391_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10391_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

diencephalon lateral wall mantle layer

weak

single cell

weak expression: see section 11 13 14

cerebral cortex ventricular layer

moderate

homogeneous

moderate expression: see section 02 03 04 05 06 07 11 12 14 15 16 17 18 19 20 21 22 weak expression: see section 01 08 09 10 23

olfactory cortex mantle layer

moderate

homogeneous

moderate expression: see section 11 12 15 16 17 18

midbrain mantle layer

weak

single cell

weak expression: see section 07 08 15 16

metanephros

weak

regional

weak expression: see section 07 20 21

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T55040
Entity Detected:	Emx1, empty spiracles homolog 1 (Drosophila) ( MGI:95387)
Sequence:	sense strand is shown >T55040 AAGGGTTCCCACCATATCAACCGGTGGCGCATCGCCACGAAGCAGGCCAACGGGGAGGACATTGATGTCA CCTCCAATGACTAGGGGAACAATCAAGAGCCCACAAGGGCAGGGCATTGCTTGCTGCTGGCCAAGGCTCC AGGGGGCCCATGCTGGACTCTGGCCACTCCTTAGCCAGGCTGCAGGGAGGCCTCGAGTCACGGCCCCACA GGGCTTGGAGCCTGGGGCCATCACTGTCAGAGGGACAAGAGAATGGGCTGGCTGAGGCCTGGGACTGCTC AGCCTTCTGGTGGAGAGCCTGCTGCTTGGGTGGACCGCCCATCACTGCAGCCCCCCAGCTGCTCTCCATG TCTCTGTCTTTGTTCTGAGACATTTCTGTTTTAATTTATTTTCCAGGTCCTGCTGTAGTTCTTAGT
Notes:	The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. Forward Primer - name:unspecified, sequence:AAGGGTTCCCACCATATCAACCG; Reverse Primer - name:unspecified, sequence:ACTAAGAACTACAGCAGGACCTGG. The reverse primer contains a 5' extension containing an unspecified RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using unspecified polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10393 same embryo
	EMAGE:10394 same embryo
	EMAGE:10390 same embryo
	EMAGE:10392 same embryo
	EurExpress:euxassay_019478 same experiment
	MGI:4824547 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source