EMAGE mouse gene expression spatial database

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EMAGE:10394

Slc5a1 solute carrier family 5 (sodium/glucose cotransporter), member 1 ( MGI:107678)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_018960. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_018960_01	euxassay_018960_02	euxassay_018960_03	euxassay_018960_04

euxassay_018960_05	euxassay_018960_06	euxassay_018960_07	euxassay_018960_08	euxassay_018960_09

euxassay_018960_10	euxassay_018960_11	euxassay_018960_12	euxassay_018960_13	euxassay_018960_14

euxassay_018960_15	euxassay_018960_16	euxassay_018960_17	euxassay_018960_18	euxassay_018960_19

euxassay_018960_20	euxassay_018960_21	euxassay_018960_22	euxassay_018960_23	euxassay_018960_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10394_wholemount_strong.wlz

10394_wholemount_moderate.wlz

10394_wholemount_weak.wlz

10394_wholemount_possible.wlz

10394_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10394_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

hindgut

moderate

regional

moderate expression: see section 14

rectum

moderate

regional

moderate expression: see section 14

midgut

moderate

regional

moderate expression: see section 13 14 15 16 17 18 19 20 weak expression: see section 12

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T51041
Entity Detected:	Slc5a1, solute carrier family 5 (sodium/glucose cotransporter), member 1 ( MGI:107678)
Sequence:	sense strand is shown >T51041 CGGACGCGTGGGGCTCGGTCTTCTTAAGGCACTGCACACCTAGGAGCTGCTTCCCGACGGTGCTGTGCAG CGCAGCAGCACAGGGGCCCAGCGTTCCTGCCGCGCAGCCCCACTGTCGCGGGTCCCCTCCCTCGGGGCTG ATCATTAACTCAAAAGCAGTATAAGGAGTGAGTGGCCCCGGTGAGCCTGTGAGCCGAGAGGGACTCGTCG CCACCGCCATGGACAGTAGCACCTTGAGCCCCGCGGTTACTGCCACCGATGCACCCATCCCGTCCTACGA GCGCATTCGCAATGCGGCTGACATCTCAGTCATCGTCATCTACTTCGTGGTGGTGATGGCTGTCGGGCTG TGGGCCATGTTTTCCACTAATCGTGGTACCGTTGGAGGCTTCTTCCTGGCAGGCCGAAGTATGGTGTGGT GGCCGATTGGAGCCTCTCTCTTTGCCAGTAACATTGGAAGTGGTCACTTTGTGGGGCTGGCAGGCACAGG AGCAGCCGCAGGCATTGCCATGGGTGGCTTTGAATGGAACGCCTTGGTTTTGGTGGTTGTGCTGGGCTGG ATATTTGTCCCGATTTACATCAAGGCCGGGGTGGTGACGATGCCGGAGTATCTGCGGAAGCGGTTTGGAG GCAAGAGAATTCAGATCTACCTTTCCGTTCTGTCCCTGCTCCTCTACATCTTCACAAAGATCTCTGCAGA CATCTTCTCCGGGGCTATATTCATCAATCTAGCCTTGGGCCTGGATATATACTTGGCCATTTTCATCCTG CTAGCAATCACTGCCCTTTATACAATCACAGGGGGGCTGGCAGCTGTAATTTACACAGATACCCTGCAGA CCGCGATCATGCTGGTGGGGTCTTTTATCCTGACTGGGTTTGCTTTCAATGAAGTAGGAGGATATGAAGC CTTCATGGACAAGTACATGAAAGCTATTCCAACTAAGGTTTCTAACGGAAATTTTACCGCCAAGGAAGAA TGCTACACACCGAGGGCTGACTCATTCCACATATTCAGAGACCCCATTACTGGAGACATGCCGTGGCCTG GGCTCATTTTTGGCCTGGCCATCCTCGCCCTGTGGTACTGGTGTACGGATCAGGTCATTGTGCAGCGATG CCTCTCGGCCAAGAACATGTCTCACGTGAAGGCTGGCTGTACCCTGTGTGGCTATCTGAAGCTACTGCCC ATGTTCCTCATGGTGATGCCAGGAATGATCAGCCGGATTCTATATACAGAAAAAATCGCCTGTGTCCTCC CTGAAGAATGTCAGAAATACTGCGGCACACCAGTGGGCTGTACCAACATCGCCTACCCCACGCTCGTGGT GGAACTCATGCCTAATGGACTGCGAGGCCTGATGCTGTCTGTCATGATGGCTTCCCTCATGAGCTCCTTG ACCTCCATCTTCAACAGCGCCAGTACTCTCTTCACCATGGACATCTACACCAAGATCCGGAAGAAGGCAT CTGAGAAGGAGCTCATGATTGCAGGAAGGTTGTTCATCCTCGTGCTGATTGGCATCAGCATTGCCTGGGT ACCCATTGTACAGTCTGCTCAAAGTGGGCAGCTTTTTGATTACATCCAGTCTATCACCAGTTACCTGGGG CCGCCCATTGCAGCTGTCTTCCTGCTTGCCATTTTCTGCAAGAGAGTCAATGAACAAGGAGCCTTCTGGG GACTGATCCTGGGCTTCCTGATTGGAATCTCCCGTATGATCACTGAGTTTGCCTATGGAACTGGGAGCTG CATGGAGCCCAGCAACTGCCCCAAGATCATCTGTGGGGTCCACTATTTGTACTTTGCCATCATCCTCTTC GTCATCAGCGTCATCACCATCTTGATCATCTCCTTCCTCACCAAGCCCATTCCAGACGTGCACCTGTACC GTTTGTGTTGGAGTCTACGCAACAGCAAGGAAGAGCGAATCGACCTGGATGCAGGAGAGGAGGAGGACAT CCCAGAGGACTCCAAGGACACAATTGAAATAGACACAGAA
Notes:	The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. Forward Primer - name:unspecified, sequence:unspecified; Reverse Primer - name:unspecified, sequence:unspecified. The reverse primer contains a 5' extension containing an unspecified RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using unspecified polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10393 same embryo
	EMAGE:10390 same embryo
	EMAGE:10392 same embryo
	EMAGE:10391 same embryo
	EurExpress:euxassay_018960 same experiment
	MGI:4828248 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source