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EMAGE:10419

Satb2 special AT-rich sequence binding protein 2 ( MGI:2679336)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:10419 EMAGE:10419 EMAGE:10419 EMAGE:10419 EMAGE:10419
"Pseudo-wholemount" of euxassay_018949. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_018949_01 euxassay_018949_02 euxassay_018949_03 euxassay_018949_04
EMAGE:10419 EMAGE:10419 EMAGE:10419 EMAGE:10419 EMAGE:10419
euxassay_018949_05 euxassay_018949_06 euxassay_018949_07 euxassay_018949_08 euxassay_018949_09
EMAGE:10419 EMAGE:10419 EMAGE:10419 EMAGE:10419 EMAGE:10419
euxassay_018949_10 euxassay_018949_11 euxassay_018949_12 euxassay_018949_13 euxassay_018949_14
EMAGE:10419 EMAGE:10419 EMAGE:10419 EMAGE:10419 EMAGE:10419
euxassay_018949_15 euxassay_018949_16 euxassay_018949_17 euxassay_018949_18 euxassay_018949_19
EMAGE:10419 EMAGE:10419 EMAGE:10419 EMAGE:10419 EMAGE:10419
euxassay_018949_20 euxassay_018949_21 euxassay_018949_22 euxassay_018949_23 euxassay_018949_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:10419Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
10419_wholemount_strong.wlz
10419_wholemount_moderate.wlz
10419_wholemount_weak.wlz
10419_wholemount_possible.wlz
10419_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:10419_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
rib
strong strong
regionalstrong expression: see section 03 04 05 06 07 08 09 10 11 21 22 23 24 weak expression: see section 12 20
humerus
strong strong
regionalstrong expression: see section 02 03 moderate expression: see section 24
fibula
strong strong
regionalstrong expression: see section 01 02
tibia
strong strong
regionalstrong expression: see section 01 02
femur
strong strong
regionalstrong expression: see section 03 04 05 06 22 23 moderate expression: see section 24
hypothalamus mantle layer
weak weak
regionalweak expression: see section 10 11 12 13 14 15
cerebral cortex mantle layer
strong strong
regionalstrong expression: see section 01 02 03 04 05 17 18 19 20 21 22 23 24 moderate expression: see section 06 07 14 15 16 weak expression: see section 08 09 13
intermediate grey horn
weak weak
regionalweak expression: see section 15 17 18
mandible
strong strong
regionalstrong expression: see section 03 04 05 06 07 08 09 10 11 14 15 16 17 18 19 20 21 22
maxilla
strong strong
regionalstrong expression: see section 03 04 05 06 07 08 09 10 11 14 15 16 17 18 19 20 21 22
palatal shelf
moderate moderate
regionalmoderate expression: see section 09 10 11 14 15 16
larynx
moderate moderate
regionalmoderate expression: see section 13 14 weak expression: see section 12
axial skeleton
strong strong
regionalstrong expression: see section 10 11 13 18 19 weak expression: see section 12
orbito-sphenoid
strong strong
regionalstrong expression: see section 01 02 03 20 21 22 23 24
clavicle
strong strong
regionalstrong expression: see section 18 19 moderate expression: see section 08 09 10 weak expression: see section 20
scapula
moderate moderate
regionalmoderate expression: see section 24
pelvic girdle skeleton
strong strong
regionalstrong expression: see section 11 18 moderate expression: see section 10 19
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T51050
Entity Detected:Satb2, special AT-rich sequence binding protein 2 ( MGI:2679336)
Sequence:sense strand is shown

>T51050
CTTCCTCAACCTGCCTGAAGTGGAGCGTGATCGCATTTACCAGGATGAGCGAGAGAGGAGCATGAACCCC
AATGTGAGCATGGTCTCCTCTGCCTCTAGCAGTCCCAGCTCCTCCCGAACCCCACAGGCCAAAACCTCGA
CACCGACAACAGACCTCCCTATTAAGGTGGACGGCGCCAACGTCAACATCACAGCTGCCATTTATGACGA
GATCCAACAGGAGATGAAAAGAGCCAAGGTGTCTCAAGCCCTGTTTGCCAAAGTGGCTGCAAACAAAAGT
CAGGGCTGGCTTTGCGAACTGCTTCGTTGGAAGGAGAACCCCAGCCCAGAAAACCGCACCCTTTGGGAGA
ATCTCTGCACCATCCGCCGTTTCCTGAATCTTCCCCAACATGAGCGGGATGTGATCTATGAGGAAGAATC
TCGACATCACCACAGTGAACGCATGCAGCATGTGGTCCAGCTCCCACCTGAGCCCGTGCAGGTCCTTCAT
CGACAGCAGTCCCAGCCAACTAAGGAGAGCTCCCCTCCCAGAGAAGAAGCACCCCCACCGCCTCCTCCAA
CAGAAGACAGCTGTGCCAAAAAGCCTCGGTCTCGCACAAAGATCTCTTTGGAAGCCCTGGGCATCCTTCA
AAGCTTCATCCATGATGTAGGCCTCTATCCCGACCAGGAAGCCATCCACACACTCTCCGCCCAGCTGGAT
CTCCCCAAACACACCATCATCAAGTTCTTCCAGAACCAGAGGTACCACGTGAAGCACCACGGGAAGCTGA
AAGAGCACCTGGGCTCCGCGGTGGACGTGGCTGAATATAAGGACGAGGAGCTGCTGACCGAGTCAGAGGA
GAAGACAGTGAGGAAGGCTCCGAGGAGATGTACAAAGTGGAGGCTGAGGAGGAAAATGCTGACAAAAGCA
AGG
Notes:The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. Forward Primer - name:unspecified, sequence:CTTCCTCAACCTGCCTGAAG; Reverse Primer - name:unspecified, sequence:CCTTGCTTTTGTCAGCAT. The reverse primer contains a 5' extension containing an unspecified RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using unspecified polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:10420 same embryo
 EurExpress:euxassay_018949 same experiment
 MGI:4827878 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS