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EMAGE:1042

Pax2 paired box gene 2 ( MGI:97486)
TS16 (10 dpc)
in situ hybridisation

Data Images
EMAGE:1042
Figs 5E&F Nornes et al, 1990 [PMID:1977575] . Copyright: This image is from Development and is displayed with the permission of the Company of Biologists Ltd who owns the copyright.

Expression pattern clarity: two stars
Find spatially similar expression patterns: Find spatially similar patterns
Notes:
Image annotations: III, third ventricle; lp, lens placode; os, optic stalk; te, telencephalon; di, diencephalon; lv, lateral ventricle.
Expression Pattern Description
Spatial Annotation:
EMAGE:1042EMAGE:1042Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
3D mapping3D context movie

View mapped 3D expression image EMAGE genex expression entry
Download individual expression domains:
1042_voxel_strong_3D_1.wlz
1042_voxel_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:1042_all_domains.zip
Find spatially similar expression patterns: EMAGE spatially similar patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
optic cup
detected detected
regionalExpression in ventral 1/2 to 2/3 of invaginating germinal epithelial layer, and ventral region of optic stalk; ends abruptly at border with diencephalon
optic stalk
detected detected
regionalExpression in ventral 1/2 to 2/3 of invaginating germinal epithelial layer, and ventral region of optic stalk; ends abruptly at border with diencephalon
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:21330
Entity Detected:Pax2, paired box gene 2 ( MGI:97486)
Notes:The probe used in this study by Nornes et al, 1990 [PMID:1977575] is described as "a unique Pax2 cDNA fragment downstream from the paired box sequence to prevent potential cross hybridization with other paired box containing genes (Dressler et al, 1990 [PMID:1977574] )". Dressler describe the probe as a "527 bp BamHI-EcoRI fragment from clone c31A". Figs 1 and 2 in Dressler et al., 1990 contain ambiguities in nucleotide numbering.
Chemistry:RNA
Strand:antisense
Label:S35
Specimen
Organism:mouse
Strain:NMRI
Age:10 dpc
Theiler Stage:TS16
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:4% paraformaldehyde
Embedding:cryosection
Staining procedure:autoradiography
General Information
Authors:Nornes et al, 1990 [PMID:1977575] Indexed by GXD, Spatially mapped by EMAGE
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:1977575] Nornes HO, Dressler GR, Knapik EW, Deutsch U, Gruss P 1990 Spatially and temporally restricted expression of Pax2 during murine neurogenesis. Development (109):797-809
 [ PMID:1977574] Dressler GR, Deutsch U, Chowdhury K, Nornes HO, Gruss P 1990 Pax2, a new murine paired-box-containing gene and its expression in the developing excretory system. Development (109):787-95
Links:MGI:2137312 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI