EMAGE mouse gene expression spatial database

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EMAGE:10445

Mrpl4 mitochondrial ribosomal protein L4 ( MGI:2137210)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_002835. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_002835_01	euxassay_002835_02	euxassay_002835_03	euxassay_002835_04

euxassay_002835_05	euxassay_002835_06	euxassay_002835_07	euxassay_002835_08	euxassay_002835_09

euxassay_002835_10	euxassay_002835_11	euxassay_002835_12	euxassay_002835_13	euxassay_002835_14

euxassay_002835_15	euxassay_002835_16	euxassay_002835_17	euxassay_002835_18	euxassay_002835_19

euxassay_002835_20	euxassay_002835_21	euxassay_002835_22	euxassay_002835_23	euxassay_002835_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10445_wholemount_strong.wlz

10445_wholemount_moderate.wlz

10445_wholemount_weak.wlz

10445_wholemount_possible.wlz

10445_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10445_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

submandibular gland primordium

moderate

regional

moderate expression: see section 07 08 09 10 11 18 19 20 21

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T994
Entity Detected:	Mrpl4, mitochondrial ribosomal protein L4 ( MGI:2137210)
Sequence:	sense strand is shown >T994 TCTCAGNCTGTTGGCCTACTGGGCTTCCCGGCACCCGGCTCTGGCTCTCCGGTGTCGGGGCTTCCGGTTG TCAAGTGGAGCTGATCCACAGCCTCAGGCTTCGGGACCTGGCGCTGATGGCGGCGGAATCGCCTTCGTGA CGTACCCGGCGCACATACGACGTCATCAGCCACCGCCGCGAGCGTCCGTACCGCAGCGACCTCGGCGCTG CGAGAGGTCCTCCGAGATGCTGCGGTTGTTCCAGGCCGCTTCCCGGGCCTCGCTTCGACTCTCTGGCTCC CGGGTCATCCACTCTCTAGCGGAAGGCGCGGAGCGGCCGGCCGAGATTTCAGAGCCGCGAGATAGCGCTG GTCTCCTAGATCCTGTTTTGCGCAAGTGTGAGCTCCGGATCCCAGTGCACCGGCGTCCGGTGCAAGCCTG GGTCGAGTCTCTCCGAGGCTTTGAGCAGGAGCGCATCGGCCTGGCGGAGCTGCACCCCGACGTGTTTGCA ACGGCGCCTCGGCTGGACATTGTGCACCAGGTCGCCATCTGGCAGAGGAACTTCAGGAGAATCAGCTATG CTAAC
Notes:	The probe template was PCR amplified from IMAGE:1972938 using vector specific primers. Forward Primer - name:T7-pME18S-FL3-fw, sequence:CGTAATACGACTCACTATAGGGCCTTCTGCTCTAAAAGCTGCG; Reverse Primer - name:T3-pME18S-FL3-rv, sequence:CAAATTAACCCTCACTAAAGGGCGACCTGCAGCTCGAGCAC. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:1972938 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10447 same embryo
	EMAGE:10446 same embryo
	EurExpress:euxassay_002835 same experiment
	MGI:4826449 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source