EMAGE mouse gene expression spatial database

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EMAGE:10449

Sh3gl1 SH3-domain GRB2-like 1 ( MGI:700010)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_000588. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_000588_01	euxassay_000588_02	euxassay_000588_03	euxassay_000588_04

euxassay_000588_05	euxassay_000588_06	euxassay_000588_07	euxassay_000588_08	euxassay_000588_09

euxassay_000588_10	euxassay_000588_11	euxassay_000588_12	euxassay_000588_13	euxassay_000588_14

euxassay_000588_15	euxassay_000588_16	euxassay_000588_17	euxassay_000588_18	euxassay_000588_19

euxassay_000588_20	euxassay_000588_21	euxassay_000588_22	euxassay_000588_23	euxassay_000588_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10449_wholemount_strong.wlz

10449_wholemount_moderate.wlz

10449_wholemount_weak.wlz

10449_wholemount_possible.wlz

10449_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10449_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

chondrocranium

weak

regional

weak expression: see section 01 02 03 04 05 06 07 08 09 22 23 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T814
Entity Detected:	Sh3gl1, SH3-domain GRB2-like 1 ( MGI:700010)
Sequence:	sense strand is shown >T814 TCTCGAGCCTGTTGGCCTACTGGAGCCGGCCGAGGTGGCTTCGGGGCGGGGGCGCAGAGCTGGCAAGCTG ATTTGGCGGTGGCGGCGCCGACCTGCGCGCGGAGGAAAGAAGTCGGTTCGGCGGCGCCGGCGGAAACCGG AGTTCGAGCGGGAGGCCTGACGGCGGCAGGCGGCATGTCGGTGGCGGGGCTGAAGAAGCAGTTCTACAAG GCGAGCCAGCTGGTCAGCGAGAAGGTTGGTGGGGCCGAAGGGACCAAACTGGATGATGACTTTAAAGATA TGGAAAAGAAGGTGGATGTCACCAGCAAGGCCGTGGCAGAGGTGCTGGTCAGAACCATAGAATATCTGCA GCCTAACCCAGCCTCGAGAGCCAAGCTGACTATGCTGAACACCGTATCCAAGATCCGGGGCCAAGTGAAG AACCCTGGCTACCCACAGTCAGAGGGTCTGTTGGGAGAGTGCATGGTTCGCCATGGCAAGGAACTAGGTG GAGAGTCCAACTTCGGTGATGCCCTGCTAGATGCAGGTGAGTCCATGAAGCGCCTGGCTGAGGTGAAGGA CTCAC
Notes:	The probe template was PCR amplified from IMAGE:1921896 using vector specific primers. Forward Primer - name:T7-pME18S-FL3-fw, sequence:CGTAATACGACTCACTATAGGGCCTTCTGCTCTAAAAGCTGCG; Reverse Primer - name:T3-pME18S-FL3-rv, sequence:CAAATTAACCCTCACTAAAGGGCGACCTGCAGCTCGAGCAC. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:1921896 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10448 same embryo
	EMAGE:10450 same embryo
	EMAGE:10451 same embryo
	EurExpress:euxassay_000588 same experiment
	MGI:4828039 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source