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EMAGE:1045

Hoxd3 homeobox D3 ( MGI:96207)
TS15 (9 dpc)
in situ hybridisation

Data Images
EMAGE:1045 EMAGE:1045
Figure 7B of Condie & Capecchi, 1993 [PMID:7910549] . Copyright: This image is from Development and is displayed with permission of The Company of Biologists Ltd. who owns the copyright. Figure 7A of Condie & Capecchi, 1993 [PMID:7910549] . Copyright: This image is from Development and is displayed with permission of The Company of Biologists Ltd. who owns the copyright.

Expression pattern clarity: two stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
A and B do not show the same embryo, (A = 9.5 dpc, B = 9.0 dpc) but both appear to be TS15. Abbreviations used: r5, rhombomere 5; lb, limb bud. The arrow in A indicates expression in the somite mesoderm. The arrow in B indicates expression in the somites.
Expression Pattern Description
Spatial Annotation:
EMAGE:1045Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
1045_wholemount_strong_3D_1.wlz
1045_wholemount_weak_3D_1.wlz
1045_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:1045_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
trunk somite
detected detected
regionalThe anterior limit of expression is between somites 4 and 5.
neural tube
detected detected
regionalThe anterior limit of expression is between rhombomeres 4 and 5. Expression caudal to rhombomere 5 is less intense than in rhombomere 5.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:1201984
Entity Detected:Hoxd3, homeobox D3 ( MGI:96207)
Sequence:sense strand is shown

>MGI:1201984
CGTGGCTTACTCCGGCCAGCTGCCGCCGGTGCCCGGCCTGGCCTACGACGCACCCTCGCCGCCCGCTTTC
GCCAAATCGCAGCCCAATATGTACGGCCTGGCCGCCTACACGGCGCCGCTCAGCAGCTGCCTGCCTCAGC
AGAAGCGGTACCCGGCGCCCGAGTTCGAGCCTCACCCCATGGCGAGCAACGGCGGCGGCTTCGCCAGCGC
CAACCTGCAGGGCAGCCCGGTGTACGTGGGCGGCAACTTCGTCGACTCCATGGCGCCCACGTCCGGGCCG
GTCTTCAATCTGGGCCACCTCTCGCACCCGTCTTCGGCCAGCGTGGACTACAGTTGCGCCGCGCAAATCC
CTGGCAACCATCACCACGGACCCTGCGACCCTCATCCCACCTACACAGATCTCTCGGCTCACCACTCGTC
TCAGGGACGCCTGCCCGAGGCCCCCAAACTGACACATCTGTAGCTGTTGCCGCCGGCCAGGCGCAATTAC
CTCTCTGGTTTTGGTGGCAGAGGTGGTGGTGGGGCGGGGCGGGGCCCGAGGGGCAGTTTAGGGGATCCCC
CTCCCTGATCTGGCCTGGCGGATGCCTCACGAGTGGCTTCCAGCGGCCAGGGCTGACGCGACTGGGCCTC
CCTCCAGGCGCGTCCTCCTTTGGGTGACTCGCCATAAATCAGCCGCAAGGATCCTCCCCAGTAAACCTGA
CAGTGCCATAAACTGCGGACCGAGGGACTCTAATCTGGTAATGGTGTCCCTAAGGTAAGTCCTCGACCCA
TCCGTGGCGAGTCCTGAAGAGGGACTAGGGCCTGGAGAACCCTGGGCCTGGCCCTTCTGTCTAGCTTAGT
TTCAGAGACCTTAATTTATAATGCTCCGTCCCCTCCTGTAAAGATTGCATCGGACTAAACAATCTGTATT
TATTATTTGAAGCGAGTAATTTCGTTTCCCTGATTATTTATCCTCGTCTTAATGTATTTATGTGTATATT
TGTAG
nt 924 - nt 1908 of NM_010468.1
Notes:The probe used in this study by Condie & Capecchi, 1993 [PMID:7910549] was a "900 bp PmlI-EcoRI fragment located immediately 3' of the homeobox".
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Age:9 dpc
Theiler Stage:TS15
Mutations:none (wild-type)
Preparation:wholemount
Procedures
General Information
Authors:Condie & Capecchi, 1993 [PMID:7910549] . Indexed by GXD, Spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:7910549] Condie BG, Capecchi MR 1993 Mice homozygous for a targeted disruption of Hoxd-3 (Hox-4.1) exhibit anterior transformations of the first and second cervical vertebrae, the atlas and the axis. Development (119):579-95
Links:MGI:1351302 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI