EMAGE mouse gene expression spatial database

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EMAGE:10484

Cpsf4 cleavage and polyadenylation specific factor 4 ( MGI:1861602)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_000876. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_000876_01	euxassay_000876_02	euxassay_000876_03	euxassay_000876_04

euxassay_000876_05	euxassay_000876_06	euxassay_000876_07	euxassay_000876_08	euxassay_000876_09

euxassay_000876_10	euxassay_000876_11	euxassay_000876_12	euxassay_000876_13	euxassay_000876_14

euxassay_000876_15	euxassay_000876_16	euxassay_000876_17	euxassay_000876_18	euxassay_000876_19

euxassay_000876_20	euxassay_000876_21	euxassay_000876_22	euxassay_000876_23	euxassay_000876_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10484_wholemount_strong.wlz

10484_wholemount_moderate.wlz

10484_wholemount_weak.wlz

10484_wholemount_possible.wlz

10484_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10484_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

submandibular gland primordium

moderate

regional

moderate expression: see section 09 17 19 20

vibrissa

weak

regional

weak expression: see section 07 08 09 23 24

telencephalon ventricular layer

moderate

regional

moderate expression: see section 02 03 04 05 06 07 08 09 10 11 12 15 16 19 20 weak expression: see section 01 13 14 17 18 21 22

midbrain ventricular layer

moderate

regional

moderate expression: see section 06 07 08 09 10 11 12 13 14

dorsal root ganglion

moderate

regional

moderate expression: see section 10 11 16 17 18 weak expression: see section 08

nasal cavity olfactory epithelium

weak

regional

weak expression: see section 10 11 12 13 16 17 18 19 20 21

kidney calyx

moderate

regional

moderate expression: see section 08 10 11 18 19 20 21

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T162
Entity Detected:	Cpsf4, cleavage and polyadenylation specific factor 4 ( MGI:1861602)
Sequence:	sense strand is shown >T162 GGCGGCTGCGGCGGCGGCGGCGGCGGCAAGCGAGAAGGGGACGGGGAGAGGTAGCGTGAGTTGCGGAGAG CGGCTGAGAGACCTGAGGGGGAGCTGGGCCCGAGGCCGCCGCCGCCGCCATGCAGGAAATAATCGCCAGC GTAGATCACATCAAGTTCGACTTGGAGATCGCCGTGGAGCAGCAGCTCGGGGCGCAGCCGCTGCCCTTCC CCGGCATGGATAAGTCAGGGGCTGCTGTCTGTGAATTCTTTTTGAAAGCCGCCTGTGGCAAAGGGGGCAT GTGTCCATTCCGCCACATCAGTGGTGAGAAGACAGTTGTGTGCAAACACTGGCTGAGAGGACTGTGCAAG AAAGGGGACCAGTGTGAGTTCTTGCATGAATATGACATGACCAAGATGCCTGAGTGCTACTTTTACTCCA AGTTCGGGGAATGCAGCAATAAGGAGTGCCCCTTCCTGCACATCGACCCTGAATCCAA
Notes:	The probe template was PCR amplified from IMAGE:2644929 using vector specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:2644929 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	MGI:4824034 same experiment
	EurExpress:euxassay_000876 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source