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EMAGE:10492

AI661453 expressed sequence AI661453 ( MGI:2146908)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:10492 EMAGE:10492 EMAGE:10492 EMAGE:10492 EMAGE:10492
"Pseudo-wholemount" of euxassay_000640. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_000640_01 euxassay_000640_02 euxassay_000640_03 euxassay_000640_04
EMAGE:10492 EMAGE:10492 EMAGE:10492 EMAGE:10492 EMAGE:10492
euxassay_000640_05 euxassay_000640_06 euxassay_000640_07 euxassay_000640_08 euxassay_000640_09
EMAGE:10492 EMAGE:10492 EMAGE:10492 EMAGE:10492 EMAGE:10492
euxassay_000640_10 euxassay_000640_11 euxassay_000640_12 euxassay_000640_13 euxassay_000640_14
EMAGE:10492 EMAGE:10492 EMAGE:10492 EMAGE:10492 EMAGE:10492
euxassay_000640_15 euxassay_000640_16 euxassay_000640_17 euxassay_000640_18 euxassay_000640_19
EMAGE:10492 EMAGE:10492 EMAGE:10492 EMAGE:10492 EMAGE:10492
euxassay_000640_20 euxassay_000640_21 euxassay_000640_22 euxassay_000640_23 euxassay_000640_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:10492Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
10492_wholemount_strong.wlz
10492_wholemount_moderate.wlz
10492_wholemount_weak.wlz
10492_wholemount_possible.wlz
10492_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:10492_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T408
Entity Detected:AI661453, expressed sequence AI661453 ( MGI:2146908)
Sequence:sense strand is shown

>T408
GCGGCGGCGCCCGCACCACCTGTCGCCGGGTGCGCGGGCGGGGAGCGCGGCTGGGGACCATGGCCTGCTA
GTGTCCCCTGGGAAGACGCTGCGGGGTTCAGTCTGGAGCAGGACAGGCCTAGGGACGGAAGGTCCTTCCC
TCGGGGACAGATCGGCCTGCAGCAGCATGAAAAAGAACCAGACCATGCAGGGCACTTTCAGCAAACTCTT
TGGGAAGAAGCACGCCCACCCCTCCACCACGTCCCTCTACGCCACCAATCCACCCTGGATTTTCACCCAC
GAGGCCCAAGAGGAGGGAACCAGGGACTTTGATGGGATCTATTATGGAGACAATCGGTTCAACACTGTGA
GCGAATCGGGAACAGCCACGCTGAAAGCCCGGCCACGAGTCCGGCCCCTGTTGACTTTCATTCCTCTGAA
TGCCCAGGAGAACCATGGGCTGGCTGTGCCCACCCCGTCTGTCCCAGAAGATTTTGCAGACAAAGAAGTG
Notes:The probe template was PCR amplified from IMAGE:3157220 using vector specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:3157220 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:10490 same embryo
 EMAGE:10495 same embryo
 EMAGE:10494 same embryo
 EMAGE:10493 same embryo
 EMAGE:10491 same embryo
 EurExpress:euxassay_000640 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS