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EMAGE:105

Shh sonic hedgehog ( MGI:98297)
TS16 (9.5 dpc)
in situ hybridisation

Data Images
EMAGE:105 EMAGE:105
Fig 2A, Epstein et al., 1999 [PMID:9847242] . Copyright: This image is from Development and is displayed with the permission of the Company of Biologists Ltd. who owns the copyright Fig 2B, Epstein et al., 1999 [PMID:9847242] . Copyright: This image is from Development and is displayed with the permission of the Company of Biologists Ltd. who owns the copyright

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Fig 2B is a section through the whole-mount hybridisation in Fig 2A. Only the wholemount data is spatially mapped in this EMAGE entry.
Expression Pattern Description
Spatial Annotation:
EMAGE:105Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
105_wholemount_strong_3D_1.wlz
105_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:105_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
diencephalon floor plate
detected detected
future midbrain floor plate
detected detected
neural tube floor plate
detected detected
telencephalon floor plate
detected detected
rhombomere 07 floor plate
detected detected
rhombomere 08 floor plate
detected detected
rhombomere 05 floor plate
detected detected
rhombomere 06 floor plate
detected detected
rhombomere 04 floor plate
detected detected
rhombomere 03 floor plate
detected detected
rhombomere 02 floor plate
detected detected
rhombomere 01 floor plate
detected detected
notochord
detected detected
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:23359
Entity Detected:Shh, sonic hedgehog ( MGI:98297)
Notes:Probe used in this study by Epstein et al., 1999 [PMID:9847242] is indicated as being originally described by Echelard et al., 1993 [PMID:7916661] ie. as "either a 2.8kb or 0.6kb RNA transcript" derived from "subclones of the original Shh cDNA inserts".
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:Swiss Webster
Age:9.5 dpc
Theiler Stage:TS16
Mutations:none (wild-type)
Preparation:wholemount plus section of wholemount
Procedures
Fixation:4% paraformaldehyde
General Information
Authors:Epstein et al., 1999 [PMID:9847242] Indexed by GXD, Spatially mapped by EMAGE
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:9847242] Epstein DJ, McMahon AP, Joyner AL 1999 Regionalization of Sonic hedgehog transcription along the anteroposterior axis of the mouse central nervous system is regulated by Hnf3-dependent and -independent mechanisms. Development (126):281-92
 [ doi:10.1016/0092-8674(93)90627-3] [ PMID:7916661] Echelard Y, Epstein DJ, St-Jacques B, Shen L, Mohler J, McMahon JA, McMahon AP 1993 Sonic hedgehog, a member of a family of putative signaling molecules, is implicated in the regulation of CNS polarity. Cell (75):1417-30
Links:MGI:1327566 same embryo
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI