EMAGE mouse gene expression spatial database

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EMAGE:10510

Rela v-rel reticuloendotheliosis viral oncogene homolog A (avian) ( MGI:103290)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_000778. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_000778_01	euxassay_000778_02	euxassay_000778_03	euxassay_000778_04

euxassay_000778_05	euxassay_000778_06	euxassay_000778_07	euxassay_000778_08	euxassay_000778_09

euxassay_000778_10	euxassay_000778_11	euxassay_000778_12	euxassay_000778_13	euxassay_000778_14

euxassay_000778_15	euxassay_000778_16	euxassay_000778_17	euxassay_000778_18	euxassay_000778_19

euxassay_000778_20	euxassay_000778_21	euxassay_000778_22	euxassay_000778_23	euxassay_000778_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10510_wholemount_strong.wlz

10510_wholemount_moderate.wlz

10510_wholemount_weak.wlz

10510_wholemount_possible.wlz

10510_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10510_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

diaphragm

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 16 17 18 19 20 21 22 23 24

limb

strong

regional

strong expression: see section 01 02 03 04 05 21 22 23 24

vertebral axis musculature

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

foregut-midgut junction

moderate

regional

moderate expression: see section 12 13 14 15 16

vibrissa

moderate

regional

moderate expression: see section 04 05 06 19 20 21 weak expression: see section 22

nasal capsule

moderate

regional

moderate expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

tongue

moderate

homogeneous

moderate expression: see section 10 11 12 13 14 15

hindgut

moderate

regional

moderate expression: see section 11 12 13 14 15 16

midgut

moderate

regional

moderate expression: see section 07 08 09 10 11 12 13 14 15 16 17 18 19 20

lower jaw incisor

moderate

regional

moderate expression: see section 06 07 08 18 19 20

lower jaw molar

moderate

regional

moderate expression: see section 08 09 10 12 13 14 15 16 17

upper jaw incisor

moderate

regional

moderate expression: see section 08 10 16 17 18 19 20

upper jaw molar

moderate

regional

moderate expression: see section 09 10 14 15 16

axial skeleton

strong

regional

strong expression: see section 07 08 09 10 11 12 13 14 15 16 17 18 19

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T1019
Entity Detected:	Rela, v-rel reticuloendotheliosis viral oncogene homolog A (avian) ( MGI:103290)
Sequence:	sense strand is shown >T1019 TCTCGAGNCTGTTGGCCTACTGGATTTTTCCGCCTCTGGCGAATGGCTTTACTTTAGCGCGNCGTGGGCT CAGCTGCGANCCCGGCCCCGCCCCCGGGACCCTGACCATGGACGATCTGTTTCCCCTCATCTTTCCCTCA GAGCCTGCCCAGGCTTCTGGGCCTTATGTGGAGATCATCGAACAGCCGAAGCAACGGGGCATGCGATTCC GCTATAAATGCGAGGGGCGCTCAGCGGGCAGTATTCCTGGCGAGAGAAGCACAGATACCACCAAGACACA CCCCACCATCAAGATCAATGGCTACACAGGACCAGGAACAGTTCGAATCTCCCTGGTCACCAAGGATCCA CCTCACCGGCCTCATCCACATGAACTTGTGGGGAAGGACTGCCGGGATGGCTACTATGAGGCTGACCTCT GCCCAGACCGCANTATCCATAGCTTCCAGAANCTGGGGATCCAGTGTGTGAAGAAGCGAGACCTGGAGCA ANCCATTAGCCNGCGANTCCAGACCAACAATAACCCCTTTCACGNTCCTATAGAGGAGCA
Notes:	The probe template was PCR amplified from IMAGE:2076600 using vector specific primers. Forward Primer - name:T7-pME18S-FL3-fw, sequence:CGTAATACGACTCACTATAGGGCCTTCTGCTCTAAAAGCTGCG; Reverse Primer - name:T3-pME18S-FL3-rv, sequence:CAAATTAACCCTCACTAAAGGGCGACCTGCAGCTCGAGCAC. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:2076600 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10509 same embryo
	EMAGE:10511 same embryo
	EMAGE:10508 same embryo
	EurExpress:euxassay_000778 same experiment
	MGI:4827690 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source