EMAGE mouse gene expression spatial database

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EMAGE:10542

Smad3 MAD homolog 3 (Drosophila) ( MGI:1201674)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_002759. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_002759_01	euxassay_002759_02	euxassay_002759_03	euxassay_002759_04

euxassay_002759_05	euxassay_002759_06	euxassay_002759_07	euxassay_002759_08	euxassay_002759_09

euxassay_002759_10	euxassay_002759_11	euxassay_002759_12	euxassay_002759_13	euxassay_002759_14

euxassay_002759_15	euxassay_002759_16	euxassay_002759_17	euxassay_002759_18	euxassay_002759_19

euxassay_002759_20	euxassay_002759_21	euxassay_002759_22	euxassay_002759_23	euxassay_002759_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10542_wholemount_strong.wlz

10542_wholemount_moderate.wlz

10542_wholemount_weak.wlz

10542_wholemount_possible.wlz

10542_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10542_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

submandibular gland primordium

moderate

regional

moderate expression: see section 07 08 09 10 18 19 20 21

vibrissa

moderate

regional

moderate expression: see section 23

telencephalon ventricular layer

weak

homogeneous

weak expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

dorsal grey horn

moderate

regional

moderate expression: see section 09 10 11 12 13 14 15 weak expression: see section 16

pharyngo-tympanic tube

moderate

regional

moderate expression: see section 01 02 03 04 23 24

esophagus

moderate

regional

moderate expression: see section 13 14

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T2364
Entity Detected:	Smad3, MAD homolog 3 (Drosophila) ( MGI:1201674)
Sequence:	sense strand is shown >T2364 CTCGAGCCAGATTCGGCACGAGGGAACTCCATCCAACTTGGTCTTGTCAAAGAACACCGATTCCACTCAA CTAAGGCACCAGCCTGTTTCTGAGACCACAGAAGAAAACCCCAGGGATGGATTTATGAACAGCTGTGTCT GCTACATACACGTGCCCCTGTCTGAAGGCCAAGTGATGGCTTCTGTTCTGGTGGCTTGAACTAACAGGTG GTGTATCGCCACCTGACTCCTTGTTTAATGACAGAGGTCTGGGATGTCACAGTCCAAAAGGAAAGTCCCT TTCTCCATGGCTGGAGTATGGAGTTTACCTTTGGAGAAGTTGTAATGGAGCATGCCCTGTCCCACCACTC TCAGAGAGGGTGTACCTGTCAAACTGGATGGCCTACATAGGTACTCCCCCCTACCCCTAGGATGCAGAGA GACGGGAACACGCCGGAGGGTAGAGCTGGGGAGAACCCATTCTTCCTTGGAAGGATCCGCTGAAGGTCAG CGTATAGGTGATGTACAGTTCCTAATATCACATCAGTCTCAGAGTGTTCACAGGAAGCAGCAAGGGCACT CGTGGAGTA
Notes:	The probe template was PCR amplified from IMAGE:1179762 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:1179762 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10546 same embryo
	EMAGE:10544 same embryo
	EMAGE:10543 same embryo
	EMAGE:10545 same embryo
	EurExpress:euxassay_002759 same experiment
	MGI:4828314 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source