EMAGE mouse gene expression spatial database

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EMAGE:10573

Ccdc93 coiled-coil domain containing 93 ( MGI:1918079)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_000793. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_000793_01	euxassay_000793_02	euxassay_000793_03	euxassay_000793_04

euxassay_000793_05	euxassay_000793_06	euxassay_000793_07	euxassay_000793_08	euxassay_000793_09

euxassay_000793_10	euxassay_000793_11	euxassay_000793_12	euxassay_000793_13	euxassay_000793_14

euxassay_000793_15	euxassay_000793_16	euxassay_000793_17	euxassay_000793_18	euxassay_000793_19

euxassay_000793_20	euxassay_000793_21	euxassay_000793_22	euxassay_000793_23	euxassay_000793_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10573_wholemount_strong.wlz

10573_wholemount_moderate.wlz

10573_wholemount_weak.wlz

10573_wholemount_possible.wlz

10573_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10573_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

diencephalon

strong

regional

strong expression: see section 12 14

midbrain floor plate

strong

homogeneous

strong expression: see section 12 14 15

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T3913
Entity Detected:	Ccdc93, coiled-coil domain containing 93 ( MGI:1918079)
Sequence:	sense strand is shown >T3913 AGTTCGGTTGTAGGGCCCGCTCTCTCCGGCTCCACATCAGCGCATCTGGATCATGGGGTTACCCAAGGGG CCAGAGGGCCAGGGACTCCCGGAGGTGGAGACGAGAGAAGACGAAGAACAGAATGTCAAGCTGACTGAAA TCCTGGAGCTCTTGGTCGCAGCTGGCTATTTCAGAGCAAGAATTAAAGGCTTGTCTCCTTTCGACAAGGT AGTAGGAGGAATGACGTGGTGTATCACCACATGCAGCTTTGATGTGGACGTGGATTTGCTCTTTCAAGAA AACTCTACAATCGGTCAGAAAATTGCTCTATCGGAAAAGATTGTCTCCGTCCTCCCAAGGATGAAGTGTC CCCACCAGCTGGAGCCCCACCAGATCCAGGGCATGGATTTTATCCACATATTCCCTGTCGTGCAGTGGCT GGTGAAACGAGCTATCGAAACAAAGGAAGAGATGGGAGACTACATCCGCTCCTACTCCATCTCTCAGTTC CAGAAGACCTACAGCCTCCCGGAGGATGATGACTTCATAAAGAGAAAAGACAAGGCCGTCAAGACGGTCG TTGGCCTCTCGGATGCTTACAAGCCCCGGCGAAAGTACCGACGGCAGCGGGGAGCAGAGGAGCTGCCAGA CGAAGAGTCCCGAGTCCACTCCACACTTCTGGAATATGGCAGGAGATACGGATTTAGTCGCCAGAGCAAA ACAGAGAAGGCTGAGGACAAGAAAACAGCACTTGCTGCAGGGCTGTCAGCTGCAGAGAAAGTGGATGCTC ACGAGGAAGATGAGCTTCAGGCAGCCGAAGAGCAGCGTATTCAGTCACTGATGACCAAGATGACCGCCAT GGCAAACGAGGAGAGCCGTCTCACCGCAAGCTCCGTGGGCCAGATCGTGGGTCTCTGCTCTGAGGAGATC AAGCAGATTGTGTCTGAGTACGCAGGGAAGCAGTCTGAGCTGTCCGCTGAAGAAAGTCCAGAGAAGTTAG GAACCTCCCAGCTGCATCAGCGGAAAGTCATTTCCTTGAATAAGCAGATTCTGCAGAAGAGCAAACATCT GGAAGAGCTGCAAGCGAATCATACCAGCCTCAAAGCCAAGTACAGCGACAGGAAGAAGACACTGACAGAG TTGAAGGATCATGGTGAGAAACTAGACAAAGAGCAGGCAGCCCTGGAGAAATTAGAAGCCAAAGCCGACC CAAGCATGCTGCAGAACCTGAGAGCACTTGTAGCCATGAATGAAAGTCTGAAAAGCCAGGAGCAGGAGTT TAAGGCACACTGCCGGGAGGAGATGGCTCGGCTACAACAGGAAATTGAGACCCTGAAAGCTGAGAGAGCA CCAGGTGAGAAGATCATCTCTGGTGGAGAGCCGCAGGGTGCCCTGACCTCCACCATGACTCACAATGAGG ACCTGGACAGACGGTACAACATGGAAAAAGAGAAGCTGTACAAGATCCGGTTACTACAGGCACGAAGAAA TCGAGAAATAGCAATTTTGCATCGCAAGATTGACGAAGTGCCCAGTCGAGCAGAGCTGATACAGTATCAG AAGCGATTTATTGAACTCTACCGTCAGATTTCCGCAGTGCACAAGGAAACCAAGCAGTTCTTCACTTTGT ACAATACCCTGGATGACAAAAAGGTTTACCTGGAGAAGGAGATTAGCCTGCTGAACTCTATTCACGAGAA CTTCTCACAAGCCATGGCCTCCCCTGCTGCCCGGGACCAGTTTTTAAGGCAGATGGAACAGATCGTCGAA GGCATTAAACAAAGCAGGATGAAGATGGAAAAGAAGAAGCAGGAGAACAAGATGAGGAGGGACCAGTTGA ACGATCAGTACTTGGAGCTCCTGGAAAAGCAGAGGCTCTACTTCAAGACTGTGAAAGAGTTCAAAGAGGA GGGCCGAAAGAATGAGCTATTACTATCTAAGATCAAAGCCAAGGCCTCCTGAAAACTCCATCTCCGTTTC TGGATGTCCCTGATATTTTTTTAATGACGTGTATAGACTACAGGATCTTGACATGGTTCTGCCGGTGATG GCAGAGAGACCCAGGAAAATTCATTTCAGTTCTCTAGACGGATGTTGTCTTTCTCCTACTTGTTTCATTT CGTCTCTCCTGGCTGCGGCTGATGGACTCAGAGCCCTACAAAGAACCGTGGGCAGTGTGGATGACACTCA GAGCCCCATAGGCAGTGCCCACTCACTCTGACCACTCAACTTCCATTTCTTCAGTGACACTGCATTTATG AAGGTACCGGATGTGTGCGGGCCCTGAGAATAAAGAGCATTATGTTCAGCAAAAAAAAAAAAAAAAAAAA AA
Notes:	The probe template was PCR amplified from IMAGE:6467830 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6467830 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10574 same embryo
	EMAGE:10572 same embryo
	EMAGE:10575 same embryo
	EurExpress:euxassay_000793 same experiment
	MGI:4823691 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source