EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:10575

Ctsd cathepsin D ( MGI:88562)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_000816. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_000816_01	euxassay_000816_02	euxassay_000816_03	euxassay_000816_04

euxassay_000816_05	euxassay_000816_06	euxassay_000816_07	euxassay_000816_08	euxassay_000816_09

euxassay_000816_10	euxassay_000816_11	euxassay_000816_12	euxassay_000816_13	euxassay_000816_14

euxassay_000816_15	euxassay_000816_16	euxassay_000816_17	euxassay_000816_18	euxassay_000816_19

euxassay_000816_20	euxassay_000816_21	euxassay_000816_22	euxassay_000816_23	euxassay_000816_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10575_wholemount_strong.wlz

10575_wholemount_moderate.wlz

10575_wholemount_weak.wlz

10575_wholemount_possible.wlz

10575_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10575_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

embryo

strong

ubiquitous

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4052
Entity Detected:	Ctsd, cathepsin D ( MGI:88562)
Sequence:	sense strand is shown >T4052 AGCGGGTCAGCTGACTCCGCGGGACTGCGGCGTCATCCTGCCTATAAGCCGGCGACCTCTGGCTTTAAGC TTTGCTCTCTTCGGGCCGCCGCGACCATGAAGACTCCCGGCGTCTTGCTGCTCATTCTCGGCCTCCTGGC TTCGTCCTCCTTCGCGATTATCAGAATCCCTCTGCGCAAGTTCACATCTATCCGTCGGACTATGACGGAG GTGGGCGGCTCTGTGGAAGACCTGATCCTCAAAGGCCCCATCACCAAGTACTCCATGCAGTCATCGCCTA AGACCACGGAGCCAGTGTCAGAGTTACTCAAAAACTACCTGGATGCCCAGTACTATGGCGATATCGGCAT CGGAACCCCGCCGCAGTGTTTCACAGTCGTCTTTGATACTGGCTCCTCTAACCTGTGGGTCCCCTCCATT CATTGCAAGATACTTGACATAGCCTGCTGGGTCCACCATAAGTACAACAGTGACAAGTCCAGCACCTATG TGAAGAACGGCACGTCCTTTGACATCCACTACGGCTCAGGAAGCCTCTCTGGGTACCTGAGCCAGGACAC TGTATCGGTTCCATGTAAGTCTGACCAGTCAAAGGCAAGAGGTATCAAGGTGGAGAAACAGATCTTTGGA GAAGCCACCAAGCAGCCTGGAATCGTATTTGTTGCAGCCAAGTTTGATGGCATCTTGGGCATGGGCTACC CTCATATCTCTGTTAACAACGTGCTTCCGGTCTTTGACAACCTGATGCAACAGAAGCTGGTGGACAAGAA CATCTTCTCCTTCTACCTGAACAGGGACCCAGAAGGGCAACCCGGAGGAGAACTAATGCTTGGTGGCACT GACTCCAAGTACTACCACGGGGAGCTGTCCTACCTGAACGTCACTCGAAAGGCCTACTGGCAGGTGCACA TGGACCAGTTGGAGGTGGGCAATGAGCTGACCCTGTGCAAGGGAGGCTGTGAGGCTATTGTGGACACAGG GACATCTCTTCTGGTGGGGCCTGTGGAAGAGGTGAAGGAGCTGCAGAAGGCCATCGGGGCAGTGCCTCTT ATCCAGGGTGAGTATATGATTCCTTGTGAGAAGGTGTCCAGCCTGCCCACGGTCTACCTGAAGCTAGGAG GCAAAAACTATGAACTACACCCAGACAAGTATATACTCAAGGTATCGCAGGGTGGAAAGACAATCTGCCT GAGTGGCTTCATGGGAATGGACATACCCCCTCCCAGTGGGCCGCTCTGGATCCTGGGCGATGTCTTCATT GGTTCCTACTACACTGTGTTTGACAGAGACAACAATAGGGTCGGCTTTGCCAATGCTGTCGTACTCTAAC TTGCTCCTTCTCCACTGTCAGGGAACTGGATCAGAGTCCAGTAGAGGAAGCCAGCCAGCCCCATCCCTCC ACCTGCCCCACTCACACATAGTCACACTCGCTTAGTGTTGCTGGACCCTTGGGGGAGACCTGGCTGGAGC TTGTCCAGCTGTTCTGTTCTGTGGTTCCTCACCCTGGGTTCAGATTGCTGCCCTCTGCCTGTCTGAAGGA GGCCAAGGCCCACCCAGTACACGAGGCTGCCTTCAAAGGCCCCTACTGGTTTAATAGCTGCTGAGATGGA TTGTCTTGTCCTGCCGCCCTTTGCTGTGTGGGCAGTACTCTGAAGCAGGCAAATGGGTCTTAGGATCCCT CCCAGAAACCTGCTCTGACCCAGACCCATCACCCAGCTTGGGGATGGCACCACGTTCTACTGCCCCTCCA ACTCTGGCCTGGCAAAGGCCTGAAGGTGAGCAGGAAGGAGCAAGAGGACAGAAGCAAAACTATGAACCTG GGGGGTTACCTAGGGCTTGACCCCGCCCTCCTGGGAAGGCATGCCTCAGCCTGGGGTAGAGGTAGGATGA CTGACTGGTTTTGGCTGGCCTCTGCTGCCCTCATCCTGGGCTAGGCAGCTGGGAGCCAAAGTTGAAATAC AAATAAAGTTGTTTTGGGCCTTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6413315 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6413315 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10573 same embryo
	EMAGE:10574 same embryo
	EMAGE:10572 same embryo
	EurExpress:euxassay_000816 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source