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EMAGE:10579

Hk1 hexokinase 1 ( MGI:96103)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:10579 EMAGE:10579 EMAGE:10579 EMAGE:10579 EMAGE:10579
"Pseudo-wholemount" of euxassay_018979. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_018979_01 euxassay_018979_02 euxassay_018979_03 euxassay_018979_04
EMAGE:10579 EMAGE:10579 EMAGE:10579 EMAGE:10579 EMAGE:10579
euxassay_018979_05 euxassay_018979_06 euxassay_018979_07 euxassay_018979_08 euxassay_018979_09
EMAGE:10579 EMAGE:10579 EMAGE:10579 EMAGE:10579 EMAGE:10579
euxassay_018979_10 euxassay_018979_11 euxassay_018979_12 euxassay_018979_13 euxassay_018979_14
EMAGE:10579 EMAGE:10579 EMAGE:10579 EMAGE:10579 EMAGE:10579
euxassay_018979_15 euxassay_018979_16 euxassay_018979_17 euxassay_018979_18 euxassay_018979_19
EMAGE:10579 EMAGE:10579 EMAGE:10579
euxassay_018979_20 euxassay_018979_21 euxassay_018979_22

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:10579Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
10579_wholemount_strong.wlz
10579_wholemount_moderate.wlz
10579_wholemount_weak.wlz
10579_wholemount_possible.wlz
10579_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:10579_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T50527
Entity Detected:Hk1, hexokinase 1 ( MGI:96103)
Sequence:sense strand is shown

>T50527
AAGGCTTGCTAGGTTAGACCAGTAACTACATCAAGGACTCCAACCCACCAGCTAGAGACCGTTCCTCAGA
CACCTGAGAAGGATCAGAAAACCCCGCTTCTGAGGACAGAAGACCTCTGGAAAAACAGTGTAGCCATCAC
CTCTACACTATGGGACAGAACTGCCAGCGAGGACAGGCTGTAGATGTTGAACCAAAAATACGACCCCCTC
TCACAGAGGAAAAGATTGATAAGTATCTGTATGCCATGCGGCTCTCTGATGAAATTCTGATAGATATCCT
GACACGCTTCAAGAAAGAGATGAAGAATGGCCTCTCCCGGGATTATAACCCAACGGCCTCCGTCAAGATG
CTGCCAACCTTTGTCCGGTCCATTCCGGACGGCTCAGAAAAGGGGGATTTCATTGCACTGGATCTCGGCG
GGTCTTCCTTTCGAATCCTGCGGGTGCAGGTGAACCACGAGAAGAGTCAGAACGTCAGCATGGAGTCTGA
GGTCTACGACACCCCAGAGAACATCGTGCACGGCAGTGGAAGCCAGCTTTTTGATCACGTCGCTGAATGC
CTCGGAGACTTCATGGAGAAAAGGAAGATCAAGGACAAGAAATTACCCGTGGGATTCACGTTTTCCTTCC
CGTGCCGACAATCCAAAATAGACGAGGCCGTACTGATCACGTGGACAAAGCGGTTCAAAGCCAGTGGCGT
GGAAGGGGCGGATGTGGTCAAGCTGCTGAATAAAGCCATTAAGAAGCGAGGGGACTATGACGCTAACATT
GTAGCTGTGGTGAATGACACAGTGGGGACCATGATGACCTGCGGCTACGATGACCAACAGTGTGAAGTCG
GCCTGATCATTGGCACTGGCACCAATGCTTGCTACATGGAGGAACTGCGACACATCGACCT
Notes:The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. Forward Primer - name:unspecified, sequence:AAGGCTTGCTAGGTTAGACC; Reverse Primer - name:unspecified, sequence:AGGTCGATGTGTCGCAGTTC. The reverse primer contains a 5' extension containing an unspecified RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using unspecified polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:10578 same embryo
 EMAGE:10580 same embryo
 EMAGE:10577 same embryo
 EMAGE:10576 same embryo
 EurExpress:euxassay_018979 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS