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EMAGE:1059

Tbx5 T-box 5 ( MGI:102541)
TS11 (EHF Downs & Davies)
in situ hybridisation

Data Images
EMAGE:1059 EMAGE:1059 EMAGE:1059 EMAGE:1059 EMAGE:1059
Figure 5D of Hochgreb et al., 2003 [PMID:13129847] Copyright: This image is from Development and is displayed with the permission of The Company of Biologists Ltd. who owns the copyright. Figure 5A of Hochgreb et al., 2003 [PMID:13129847] Copyright: This image is from Development and is displayed with the permission of The Company of Biologists Ltd. who owns the copyright. Figure 5B of Hochgreb et al., 2003 [PMID:13129847] Copyright: This image is from Development and is displayed with the permission of The Company of Biologists Ltd. who owns the copyright. Figure 5C of Hochgreb et al., 2003 [PMID:13129847] Copyright: This image is from Development and is displayed with the permission of The Company of Biologists Ltd. who owns the copyright. Figure 5E of Hochgreb et al., 2003 [PMID:13129847] Copyright: This image is from Development and is displayed with the permission of The Company of Biologists Ltd. who owns the copyright.

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Tbx5 expression is shown in purple whereas Aldh1a2 expression is shown in orange. ***Only the Tbx5 signal has been spatially mapped here.*** All figures shown are from the same study and are all TS11. The chronological order of development across TS11 is A, B, C, D and then E. Only figure D was spatially mapped though as it is the closest morphological match to the EMAP TS11 model (#1). Image annotations: LB - late bud; EHF - earlyhead fold; white arrowhead indicates overlap of expression in the cardiac precursors.
Expression Pattern Description
Spatial Annotation:
EMAGE:1059Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
1059_wholemount_strong_3D_1.wlz
1059_wholemount_moderate_3D_1.wlz
1059_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:1059_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
cardiogenic plate
detected detected
regionalExpression was detected in the cardiac precursors.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:1340309
Entity Detected:Tbx5, T-box 5 ( MGI:102541)
Notes:The Tbx5 probe used in this study by Hochgreb et al., 2003 [PMID:13129847] was previously described by Bruneau et al., 1999 [PMID:10373308] but is of undefined sequence.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:FVB/N
Age:EHF Downs & Davies
Theiler Stage:TS11
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
Staining procedure:alkaline phosphatase + BMpurple
General Information
Authors:Hochgreb et al., 2003 [PMID:13129847] Indexed by GXD, Spatially Mapped by EMAGE
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1242/dev.00750] [ PMID:13129847] Hochgreb T, Linhares VL, Menezes DC, Sampaio AC, Yan CY, Cardoso WV, Rosenthal N, Xavier-Neto J 2003 A caudorostral wave of RALDH2 conveys anteroposterior information to the cardiac field. Development (130):5363-74
 [ doi:10.1006/dbio.1999.9298] [ PMID:10373308] Bruneau BG, Logan M, Davis N, Levi T, Tabin CJ, Seidman JG, Seidman CE 1999 Chamber-specific cardiac expression of Tbx5 and heart defects in Holt-Oram syndrome. Dev Biol (211):100-8
Links:MGI:2684667 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI