EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:10614

Slc16a14 solute carrier family 16 (monocarboxylic acid transporters), member 14 ( MGI:1919031)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_000858. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_000858_01	euxassay_000858_02	euxassay_000858_03	euxassay_000858_04

euxassay_000858_05	euxassay_000858_06	euxassay_000858_07	euxassay_000858_08	euxassay_000858_09

euxassay_000858_10	euxassay_000858_11	euxassay_000858_12	euxassay_000858_13	euxassay_000858_14

euxassay_000858_15	euxassay_000858_16	euxassay_000858_17	euxassay_000858_18	euxassay_000858_19

euxassay_000858_20	euxassay_000858_21	euxassay_000858_22	euxassay_000858_23	euxassay_000858_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10614_wholemount_strong.wlz

10614_wholemount_moderate.wlz

10614_wholemount_weak.wlz

10614_wholemount_possible.wlz

10614_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10614_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

diencephalon lateral wall mantle layer

weak

homogeneous

weak expression: see section 08 09 10 11 12 13 14 15 16 17 18 19

telencephalon mantle layer

moderate

homogeneous

moderate expression: see section 02 03 04 05 weak expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

telencephalon ventricular layer

moderate

homogeneous

moderate expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 16 17 18 19 20 21 22 23 24

medulla oblongata alar plate mantle layer

weak

homogeneous

weak expression: see section 09 10 15 16 17 18 19 20 not examined expression: see section 13

medulla oblongata basal plate mantle layer

weak

homogeneous

weak expression: see section 09 10 11 12 13 14 15 16 17 18 19 20

cerebellum intraventricular portion mantle layer

weak

homogeneous

weak expression: see section 05 06 07 08 18 19 20 21 22 23

pons mantle layer

moderate

homogeneous

moderate expression: see section 18 19 weak expression: see section 08 09 10 11 12 13 15 17

midbrain mantle layer

weak

homogeneous

weak expression: see section 07 08 09 10 11 12 13 15 16 17 18 19 20 21

midbrain ventricular layer

moderate

homogeneous

moderate expression: see section 10 11 12 13 15 16 17 18 not examined expression: see section 19

spinal cord mantle layer

moderate

homogeneous

moderate expression: see section 13 14 15 16 17 18

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T991
Entity Detected:	Slc16a14, solute carrier family 16 (monocarboxylic acid transporters), member 14 ( MGI:1919031)
Sequence:	sense strand is shown >T991 TCTCGAGNCTGTTGGCCTACTGGCTGCAGGATGCAGGTCGACATTGGCACCAATCCCCATGCCCAGCGAC CCTGGGTTCTCCGAGCGGACCTGCAAGTTCACAGGTCGAGACTGGGAGCCGAGTGAGATCTTAGCCTCTC GGGCTGCGTGCAAAAACCTTAGAAAATGTATACGAGTCATGAAGATATCGGGTATGACCTTGAAGACGAC CGAAAGGCTAAGAATAAGAAGACGCTAAAGCCCCACCCAGATATCGATGGCGGGTGGGCCTGGATGATGG TCCTCTCGTCCTTCTTTGTTCACATCCTCATCATGGGCTCACAGATGGCCCTAGGAGTCCTCAACGTGGA GTGGCTTGAAGAGTTTCACCAGAGCCGCGGCCTGACCGCCTGGGTCAGCTCCCTGAGCATGGGCATCACC TTGATCGTGGGACCTTTCATCGGCTTGTTCATTAACACCTGCGGGTGCCGCCAGACAGCAATCATTGGAG GCCTGGTGAACTCCCTCGGCTGGGTGTTGAGCGCCTATGCAGCCAATGTGCAGTCTCTCTTTA
Notes:	The probe template was PCR amplified from IMAGE:1972841 using vector specific primers. Forward Primer - name:T7-pME18S-FL3-fw, sequence:CGTAATACGACTCACTATAGGGCCTTCTGCTCTAAAAGCTGCG; Reverse Primer - name:T3-pME18S-FL3-rv, sequence:CAAATTAACCCTCACTAAAGGGCGACCTGCAGCTCGAGCAC. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:1972841 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10617 same embryo
	EMAGE:10616 same embryo
	EMAGE:10615 same embryo
	EurExpress:euxassay_000858 same experiment
	MGI:4828110 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source