EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:10615

Tctn2 tectonic family member 2 ( MGI:1915228)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_000837. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_000837_01	euxassay_000837_02	euxassay_000837_03	euxassay_000837_04

euxassay_000837_05	euxassay_000837_06	euxassay_000837_07	euxassay_000837_08	euxassay_000837_09

euxassay_000837_10	euxassay_000837_11	euxassay_000837_12	euxassay_000837_13	euxassay_000837_14

euxassay_000837_15	euxassay_000837_16	euxassay_000837_17	euxassay_000837_18	euxassay_000837_19

euxassay_000837_20	euxassay_000837_21	euxassay_000837_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10615_wholemount_strong.wlz

10615_wholemount_moderate.wlz

10615_wholemount_weak.wlz

10615_wholemount_possible.wlz

10615_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10615_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

3rd ventricle choroid plexus

strong

homogeneous

strong expression: see section 12

lateral ventricle choroid plexus

strong

homogeneous

strong expression: see section 07 08 09 10 11 14 15 16 17 18 19

telencephalon ventricular layer

weak

homogeneous

weak expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

4th ventricle

strong

homogeneous

strong expression: see section 05 06 07 09 10 11 12 13 14 15 16 17 18 19 20 21 22

not examined

homogeneous

not examined expression: see section 15

medulla oblongata basal plate ventricular layer

weak

homogeneous

weak expression: see section 11 12 13 14 15

not examined

homogeneous

not examined expression: see section 04 05 06 07 08 16 17 18 19 20 21 22

rest of cerebellum ventricular layer

weak

homogeneous

weak expression: see section 04 05 06 07 08 09 10 11 16 17 18 19 20 21 22

not examined

homogeneous

not examined expression: see section 04

pons ventricular layer

weak

homogeneous

weak expression: see section 09 10 11 12 13 16 17 18 not examined expression: see section 19 20 21 22

midbrain ventricular layer

weak

homogeneous

weak expression: see section 10 11 12 13 14 15 16

viscerocranium

moderate

regional

Expression in the turbinate bone.

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T985
Entity Detected:	Tctn2, tectonic family member 2 ( MGI:1915228)
Sequence:	sense strand is shown >T985 CGCCCTTTTTCTCTTCNGGCTATTCTGGGTTTCTGAGGCAGGCCGGCCCGGGTATGGGCTCTCTGTCACC GCTCAGCCTCCTGTGGGGACTCCTCCTGCTGCAGGGCGTCCTGAGGCCGCTGCGCGGGGACCCGGTTTTC ATCCCACCTTTCATCCGGATGTCCAGCCCTGAGGTCCGAGCGACCCTGGTCGGAGGCAGCGAGGATGTCG CTGTGTCTCTGAGTCTGCTGCAGGTCGAGGAAGGAGTGTTGCCAGTTCCTACTTGTGGCGGCCGCCGCAA TGAGACAGTGGATTGGAATGTGACTGTGAGCCCCCGAGAGAGCACGCTGGAAGTGACCATAAGGTGGAAG AGGGGTCTAGACTGGTGCTCCGCAGACGAGACAGCCTCATTCTCAGAGCCCCCGTGTGTTCTGCAGATGC TGCTAGTTTCAGCCTCCCACAATGCATCCTGCTTAGCACATCTGCTCATCCAGGTGGAGATTTATCCCAA CACATCTGTAACCCACA
Notes:	The probe template was PCR amplified from IMAGE:1972566 using vector specific primers. Forward Primer - name:T7-pME18S-FL3-fw, sequence:CGTAATACGACTCACTATAGGGCCTTCTGCTCTAAAAGCTGCG; Reverse Primer - name:T3-pME18S-FL3-rv, sequence:CAAATTAACCCTCACTAAAGGGCGACCTGCAGCTCGAGCAC. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:1972566 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10617 same embryo
	EMAGE:10616 same embryo
	EMAGE:10614 same embryo
	EurExpress:euxassay_000837 same experiment
	MGI:4828650 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source